Simpósio e Workshop destacam doenças bacterianas e fúngicas
Cientistas brasileiros e estrangeiros estão reunidos para discutir os avanços na área, incluindo temas como inovações em biotecnologia, resistência a antibióticos e qualidade de vida
Inovações em biotecnologia, interações patógeno-hospedeiro, resistência a antibióticos e qualidade de vida são temas que norteiam o III Simpósio e IV Workshop em Pesquisa, Desenvolvimento e Inovação em Doenças Bacterianas e Fúngicas, promovido pelo Instituto Oswaldo Cruz (IOC/Fiocruz). Voltado para pesquisadores, estudantes de graduação e pós-graduação e profissionais que atuam na área, o encontro é realizado nos dias 05 e 06 de novembro, das 09h às 17h, no auditório do Museu da Vida, no campus da Fiocruz, em Manguinhos (Av. Brasil, 4.365 - Rio de Janeiro).
De acordo com a pesquisadora do Laboratório de Microbiologia Celular do IOC, Maria Helena Saad, o objetivo do encontro é dar visibilidade aos estudos realizados na área, como o de biomarcadores – indicadores que podem auxiliar no diagnóstico e na compreensão da relação patógeno-hospedeiro. “Compreender melhor os mecanismos pelos quais fungos e bactérias modulam o sistema imune ou metabólico dos hospedeiros contribui para pesquisas no campo da prevenção e do diagnóstico”, destacou Maria Helena, uma das organizadoras do Simpósio e Workshop. O Simpósio oferece também oportunidade para discutir aspectos relacionados à resistência dos microrganismos aos quimioterápicos associada ao tratamento e à sua veiculação através do meio ambiente.
A programação, que integra as comemorações dos 115 anos do IOC, conta com conferências, mesas-redondas e sessões de pôsteres. O evento recebe cientistas reconhecidos internacionalmente, como Dany Beste, da University of Surrey, da Inglaterra; Frank Pessler, do Helmholtz Centre for Infection Research, da Alemanha; Guilhem Janbon, do Instituto Pasteur, da França; e Bernard Larouze, da Universidade Pierre e Marie Curie, da França.
Confira a programação:
NOVEMBER 05
8h | Registration, badges, materials and poster display
8h30 | Opening ceremony
9h | Opening lecture: Defining the metabolic phenotype of intracellular Mycobacterium tuberculosis, Dany Beste (University of Surrey/Inglaterra)
Abstract: Intracellular carbon metabolism has emerged as an attractive drug target yet the major carbon sources of intracellularly replicating pathogens remain poorly defined. This is particularly pertinent for the tuberculosis bacillus Mycobacterium tuberculosis which causes a long-term latent infection in one third of the world’s population and is resistant to many drugs. Here, we use a novel systems-based approach, 13C-flux spectral analysis (FSA) to measure, for the first time, the metabolic interaction between M. tuberculosis and its macrophage host cell. 13C-FSA analysis of experimental data showed that M. tuberculosis obtains a mixture of amino acids, C1 and C2 substrates from its host cell. The C2 source is consistent with acetate derived from host lipid catabolism and we experimentally confirmed that the C1 substrate was derived from CO2. 13C labelling experiments performed on a phosphoenolpyruvate carboxykinase (PEPCK) deletion mutant demonstrated a deficiency in gluconeogenesis of the C2 and C1 substrates and thereby revealed the additional uptake and metabolism of a host-derived C3 glycolytic compound by intracellular M. tuberculosis. The finding that intracellular M. tuberculosis has access to diverse carbon sources within a macrophage provides new opportunities for development of novel chemotherapeutics that target nutrient uptake and/or intracellular metabolism of the TB bacillus.
Coordinator: Hugo Caire de Castro Faria Neto (IOC/Fiocruz)
10h | Coffee-break
Biomarkers
10h15 | Lecture: RNA molecules as biomarkers for infectious diseases, Frank Pessler (HZI-Twincore/Alemanha)
Abstract: MiRNAs fulfil nearly all requisites for a successful biomarker: they play physiologicall important roles in diverse biological processes, they are present and stable in a variety of body fluids, and they have already been proven to be good discriminators between pathological and physiological states. This is why we decided to focus our efforts on developing miRNAs and other small non-coding RNAs into clinically useful biomarkers. The first two examples to be discussed are derived from well characterized animal models of influenza A virus infection, whereas the third example is taking from a study on biomarkers for latent TB infection right here in Rio.
Coordinator: Danuza Esquenazi (IOC/Fiocruz)
Moderator: Guilhem Janbon (Institut Pasteur/França)
11h15 | Lecture: Introns regulate gene expression in Cryptococcus neoformans, Guilhem Janbon (Institut Pasteur/França)
Abstract: Cryptococcus neoformans is a basidiomycetous opportunistic pathogen leaving in the environment responsible for more than 500 000 deaths every years toward to the globe. Our recent work suggests that a fascinating, complex pattern of RNA molecules composes its transcriptome and this fungus is emerging for different aspects as an ideal model to study RNA metabolism in eukaryotes. It is also tempting to hypothesize that this complex RNA metabolism provides a mechanism for this yeast to respond to different environmental cues and to be an efficient pathogen. The most prominent features of this transcriptome are introns. Our recent re-annotation of the C. neoformans genome revealed that nearly all the expressed genes contain intron. They can be present within the coding sequence but also within UTR regions. We also demonstrated that these introns are essential for gene expression. We also identified a large number of alternative splicing events. However, alternative splicing in this yeast seems to be more a mean to regulate gene expression than to diversify the proteome.
Coordinator: Marcio Lourenço Rodrigues (CDTS/Fiocruz)
Moderator: Frank Pessler (HZI-Twincore/Alemanha)
12h15 | Lunch
13h30 | E-Poster (oral presentation)
14h30 | Round table
Coordinator: Roberta Olmo (IOC/Fiocruz)
Moderator: Geraldo Pereira (IOC/Fiocruz)
Speakers: Leonardo da Silva Araújo (IOC/Fiocruz), Novel antigen-specific T-cells markers to Mycobacterium tuberculosis infection; Nelson Alves Junior (UFRJ), O uso de sequenciamento de nova geração para estudo de diversidade e função de micro-organismos; Milton Ozório (IOC/Fiocruz), Molecular and genetic signatures for detection of disease progression in tuberculosis and leprosy; Adalberto Resende (IOC/Fiocruz), A farmacogenética na tuberculose: possíveis aplicações
15h50 | Coffee-break
16h | Lecture: Biomarkers in TB research: contributions from the study of BCG and other mycobacteria, Leila Mendonça Lima (IOC/Fiocruz)
Coordinator: Euzenir Sarno (IOC/Fiocruz)
Moderator: Dany Beste (University of Surrey/Inglaterra)
NOVEMBER 06
Virulence and host-pathogen relationship
9h | Lecture: A importância de MAP quinases e fosfatases na virulência de Aspergillus fumigatus, Gustavo Goldman (USP)
Abstract: Aspergillus fumigatus is a fungal pathogen that causes several invasive and noninvasive diseases named aspergillosis. This disease is generally regarded as multifactorial, considering that several pathogenicity determinants are present during the establishment of this illness. It is necessary to obtain an increased knowledge of how, and which, A. fumigatus signal transduction pathways are engaged in the regulation of these processes. Protein phosphatases are essential to several signal transduction pathways. We identified 32 phosphatase catalytic subunit-encoding genes in A. fumigatus, of which we were able to construct 24 viable deletion mutants. The role of nine phosphatase mutants in the HOG (high osmolarity glycerol response) pathway was evaluated by measuring phosphorylation of the p38 MAPK (SakA) and expression of osmo-dependent genes. We were also able to identify 11 phosphatases involved in iron assimilation, six that are related to gliotoxin resistance, and three implicated in gliotoxin production. These results present the creation of a fundamental resource for the study of signaling in A. fumigatus and its implications in the regulation of pathogenicity determinants and virulence in this important pathogen.
Coordinator: Leon Rabinovitch (IOC/Fiocruz)
Moderator: Maria Cristina Pessolani (IOC/Fiocruz)
10h | Coffee-break
10h15 | Round table I
Coordinator: Lucimar Kneipp (IOC/Fiocruz)
Moderator: André Luís Souza dos Santos (UFRJ)
Speakers: Leonardo Nimrichter (UFRJ), Atividade imunobiológica de vesículas extracelulares produzidas por fungos; Alex Guerra Ferreira (UERJ), Biofilmes bacterianos: mais do que um fator de virulência; Vera Lúcia Garcia Calish (USP), Innate Immunity in Pulmonary Parcoccidioidomycosis
11h10 | Round table II
Coordinator: Maria Cristina Pessolani (IOC/Fiocruz)
Moderator: Gustavo Goldman (USP)
Speakers: Fernanda Lopes Fonseca (CDTS/Fiocruz), Metabolismo de quitina e patogênese no gênero Cryptococcus; Sandro Rogério Almeida (USP), Basis of cellular immune response in chromoblastomycosis and implications for therapy; Douglas Mcintosh (UFRRJ), The secret life of a commensal yeast-trying to understand the genus Cyniclomyces
12h05 | Lunch
13h05 | E-poster
Resistance: reservoir and environment
14h05 | Lecture: Disseminação da resistência aos antimicrobianos: da clínica para a comunidade ou da comunidade para a clínica?, Renata Cristina Picão (UFRJ)
Coordinator: Viviane Zahner (IOC/Fiocruz)
Moderator: Douglas Mcintosh (UFRRJ)
15h05 | Coffee-break
15h20 | Round table
Coordinator: Ana Luiza Guaraldi (UERJ)
Moderator: Ana Paula Assef (IOC/Fiocruz)
Speakers: Bernard Larouze (UPMC-Paris - ENSP/Fiocruz), Tuberculosis in a Brazilian prison; Polyana Pereira (UFRJ), Overview of bacterial resistance in the hospital environment; Thiago Pavoni (IOC/Fiocruz), The role of hospital wastewater in the spread of antimicrobial-resistant bacteria to the community.
16h30 | Awards/Closing session
Organizing Committee
President
Maria Helena Féres Saad
Members
Lucimar Ferreira Kneipp (IOC/Fiocruz)
Viviane Zahner (IOC/Fiocruz)
Scientific Committee
Lucimar Ferreira Kneipp (IOC/Fiocruz)
Maria Helena Feres Saad (IOC/Fiocruz)
Viviane Zahner (IOC/Fiocruz)
Coordinators of poster evaluation
Aurea Moraes (IOC/Fiocruz)
Cátia Sodré (UFF)
Danuza Esquenazi (IOC/Fiocruz)
Gisela Lara (IOC/Fiocruz)
Flavio Alves Lara (IOC/Fiocruz)
Manuel Marques Evangelista de Oliveira (INI/Fiocruz)
Letícia Miranda Lery Santos (IOC/Fiocruz)
Renata Costa (IOC/Fiocruz)
Roberta Olmo (IOC/Fiocruz)
Karyne Rangel (INCQS/Fiocruz)
Luciane Martins Medeiros (Biomanguinhos/Fiocruz)
01/07/2015 - Atualizado em 04/11/2015
Lucas Rocha
Autorizada a reprodução sem fins lucrativos desde que citada a fonte (Comunicação / Instituto Oswaldo Cruz).
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