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Área de PDI em Pesquisa, Desenvolvimento e Inovação em Helmintoses 2013

Veja, abaixo, a relação de artigos científicos publicados pelo IOC, na referida Área Temática, organizados em ordem alfabética crescente: 

Total: 0
Andrade JG, Iniguez AM, Souza AN, Marques VCL, de Souza GA, Santos CP and DaMatta RA (2013), "Genetic characterization of the blood-sucking nematodes Libyostrongylus dentatus and Libyostrongylus douglassii supports their different evolutionary history", Veterinary Parasitology., March, 2013. Vol. 193(1-3), pp. 193-199. Elsevier Science Bv.
Abstract: Libyostrongylus sp. are nematodes that infect ostriches. Libyostrongylus douglassii was first described in ostriches from several countries in the world. Later Libyostrongylus dentatus was morphologically identified in ostriches in the USA and Brazil, and mixed infection is common in the latter country. The internal transcribed spacer (ITS) region of the ribosomal DNA gene is used for genetic variability assessment and phylogenetic reconstruction for many organisms. Through genetic analysis the status of different species morphologically defined was confirmed and a molecular method was developed to differentiate both species. ITS1, 5.8S, ITS2 regions of L. douglassii and L dentatus were characterized. Regarding complete ITS region, the K2-p genetic distance between the species was 0.060 (SE 0.008) and the intra-specific distance was 0.002 (SE0.001) for L. dentatus and 0.006 (SE 0.002) for L.douglassii. NJ and MP phylogenetic analysis of ITS1 and ITS2 regions indicated that both species belong to the Trichostrongylidae family, and are evolutionarily different, suported by high bootstrap value. Based on ITS DNA polymorphisms, a molecular approach was designed to detect both species. These results are the first molecular characterization of L. douglassii and L dentatus, and provide new tools for the identification of these parasites of veterinary importance. (C) 2012 Elsevier B.V. All rights reserved.
BibTeX:
 @article{Andrade2013, author = {Andrade, J. G. and Iniguez, A. M. and Souza, A. N. and Marques, V. C. L. and de Souza, G. A. and Santos, C. P. and DaMatta, R. A.}, title = {Genetic characterization of the blood-sucking nematodes Libyostrongylus dentatus and Libyostrongylus douglassii supports their different evolutionary history}, journal = {Veterinary Parasitology}, publisher = {Elsevier Science Bv}, year = {2013}, volume = {193}, number = {1-3}, pages = {193--199}, doi = {10.1016/j.vetpar.2012.11.024} } 
Camacho M, Pessanha T, Leles D, Dutra JMF, Silva R, de Souza SM and Araujo A (2013), "Lutz's spontaneous sedimentation technique and the paleoparasitological analysis of sambaqui (shell mound) sediments.", Mem Inst Oswaldo Cruz., Apr, 2013. Vol. 108(2), pp. 155-159.
Abstract: Parasite findings in sambaquis (shell mounds) are scarce. Although the 121 shell mound samples were previously analysed in our laboratory, we only recently obtained the first positive results. In the sambaqui of Guapi, Rio de Janeiro, Brazil, paleoparasitological analysis was performed on sediment samples collected from various archaeological layers, including the superficial layer as a control. Eggs of Acanthocephala, Ascaridoidea and Heterakoidea were found in the archaeological layers. We applied various techniques and concluded that Lutz's spontaneous sedimentation technique is effective for concentrating parasite eggs in sambaqui soil for microscopic analysis.
BibTeX:
 @article{Camacho2013, author = {Camacho, Morgana and Pessanha, Thaíla and Leles, Daniela and Dutra, Juliana M F. and Silva, Rosângela and de Souza, Sheila Mendonça and Araujo, Adauto}, title = {Lutz's spontaneous sedimentation technique and the paleoparasitological analysis of sambaqui (shell mound) sediments.}, journal = {Mem Inst Oswaldo Cruz}, year = {2013}, volume = {108}, number = {2}, pages = {155--159}, doi = {10.1590/0074-0276108022013005} } 
Cavalcanti MG, Silva LF, Peralta RHS, Barreto MGM and Peralta JM (2013), "Schistosomiasis in areas of low endemicity: a new era in diagnosis", Trends In Parasitology., February, 2013. Vol. 29(2), pp. 75-82. Elsevier Sci Ltd.
Abstract: Parasitological detection of Schistosoma is the cornerstone of schistosomiasis diagnosis in areas of transmission worldwide. However, a steep decrease of sensitivity in low-endemicity areas (LEAs) compromises estimation of schistosomiasis. Despite the restricted utilization of molecular and immunodiagnostic techniques, recent improvements and advances have been contributing to change this scenario, especially in LEAs. Nonetheless, the main issue in a new era of diagnosis overcomes technical advances per se and relates to the loss of 'gold standards' in schistosomiasis diagnosis in LEAs. Here, we review and discuss the current role of molecular and immunodiagnostic methods in schistosomiasis management.
BibTeX:
 @article{Cavalcanti2013, author = {Cavalcanti, M. G. and Silva, L. F. and Peralta, R. H. S. and Barreto, M. G. M. and Peralta, J. M.}, title = {Schistosomiasis in areas of low endemicity: a new era in diagnosis}, journal = {Trends In Parasitology}, publisher = {Elsevier Sci Ltd}, year = {2013}, volume = {29}, number = {2}, pages = {75--82}, doi = {10.1016/j.pt.2012.11.003} } 
De Oliveira RB, Senger MR, Vasques LM, Gasparotto J, dos Santos JPA, Pasquali MAD, Moreira JCF, Silva FP and Gelain DP (2013), "Schistosoma mansoni infection causes oxidative stress and alters receptor for advanced glycation endproduct (RAGE) and tau levels in multiple organs in mice", International Journal For Parasitology., April, 2013. Vol. 43(5), pp. 371-379. Elsevier Sci Ltd.
Abstract: Schistosomiasis is a parasitic disease caused by trematode worms from the Schistosoma genus and is characterized by high rates of morbidity. The main organs affected in this pathology, such as liver, kidneys and spleen, are shifted to a pro-oxidant state in the course of the infection. Here, we compared oxidative stress parameters of liver, kidney and spleen with other organs affected by schistosomiasis - heart, brain cortex and lungs. The results demonstrated that mice infected with Schistosoma mansoni had altered non-enzymatic antioxidant status in lungs and brain, increased carbonyl levels in lungs, and a moderate level of oxidative stress in heart. A severe redox imbalance in liver and kidneys and decreased non-enzymatic antioxidant capacity in spleen were also observed. Superoxide dismutase and catalase activities were differently modulated in liver, kidney and heart, and we found that differences in Superoxide dismutase 2 and catalase protein content may be responsible for these differences. Lungs had decreased receptor for advanced glycation endproduct expression and the brain cortex presented altered tau expression and phosphorylation levels, suggesting important molecular changes in these tissues, as homeostasis of these proteins is widely associated with the normal function of their respective organs. We believe that these results demonstrate for the first time that changes in the redox profile and expression of tissue-specific proteins of organs such as heart, lungs and brain are observed in early stages of S. mansoni infection. (C) 2013 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
BibTeX:
 @article{Oliveira2013, author = {De Oliveira, R. B. and Senger, M. R. and Vasques, L. M. and Gasparotto, J. and dos Santos, J. P. A. and Pasquali, M. A. D. and Moreira, J. C. F. and Silva, F. P. and Gelain, D. P.}, title = {Schistosoma mansoni infection causes oxidative stress and alters receptor for advanced glycation endproduct (RAGE) and tau levels in multiple organs in mice}, journal = {International Journal For Parasitology}, publisher = {Elsevier Sci Ltd}, year = {2013}, volume = {43}, number = {5}, pages = {371--379}, doi = {10.1016/j.ijpara.2012.12.006} } 
De Siqueira NG, de Siqueira CMVM, Rodrigues-Silva R, Soares MDCP and Povoa MM (2013), "Polycystic echinococcosis in the state of Acre, Brazil: contribution to patient diagnosis, treatment and prognosis", Memorias Do Instituto Oswaldo Cruz., August, 2013. Vol. 108(5), pp. 533-+. Fundaco Oswaldo Cruz.
Abstract: The lack of knowledge regarding polycystic hydatid disease results in delayed or even incorrect diagnosis. The lack of systematic information regarding treatment also makes it difficult to assess the results and prognosis in patients with peritoneal and hepatic lesions caused by Echinococcus vogeli. Here we describe the clinical features of patients, propose a radiological classification protocol and describe a therapeutic option for the treatment of hydatid disease that previously had only been used for cases of cystic echinococcosis (Echinococcus granulosus). A prospective cohort study was initiated in 1999 and by 2009 the study included 60 patients. These patients were classified according to the PNM classification (parasite lesion, neighbouring organ invasion and metastases) and placed in one of three therapeutic modalities: (i) chemotherapy with albendazole at a dose of 10 mg/kg/day, (ii) surgical removal of cysts or (iii) percutaneous puncture of the cysts via puncture, aspiration, injection and re-aspiration (PAIR). The results were stratified according to therapeutic outcome: "cure", "clinical improvement", "no improvement", "death" or "no information". The PNM classification was useful in indicating the appropriate therapy in cases of polycystic hydatid disease. In conclusion, surgical therapy produced the best clinical results of all the therapies studied based on "cure" and "clinical improvement" outcomes. The use of PAIR for treatment requires additional study.
BibTeX:
 @article{Siqueira2013, author = {De Siqueira, N. G. and de Siqueira, C. M. V. M. and Rodrigues-Silva, R. and Soares, M. D. C. P. and Povoa, M. M.}, title = {Polycystic echinococcosis in the state of Acre, Brazil: contribution to patient diagnosis, treatment and prognosis}, journal = {Memorias Do Instituto Oswaldo Cruz}, publisher = {Fundaco Oswaldo Cruz}, year = {2013}, volume = {108}, number = {5}, pages = {533--+}, doi = {10.1590/0074-0276108052013001} } 
Faro MJ, Perazzini M, Corrêa LDR, Mello-Silva CC, Pinheiro J, Mota EM, de Souza S, de Andrade Z and Júnior AM (2013), "Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni.", Exp Parasitol., Jun, 2013. Vol. 134(2), pp. 228-234.
Abstract: Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the parasitic castration of Biomphalaria glabrata infected with Schistosoma mansoni during both the pre-patent and patent periods. The effect of infection on snail fecundity and fertility, growth rate and survival was studied during the 62days following miracidia exposure. An integrated approach was employed that used biochemical and histological tools over the same period. To study the effect of infection on reproduction, we individually exposed 30 snails to 5 miracidia each and tracked their fertility and fecundity. For our histopathological studies, 50 snails were exposed to 20 miracidia each, and for our histochemical studies, 50 snails were exposed to 5 miracidia each. An equal number of uninfected snails were used as a control for each group. The B. glabrata exposed to the BH strain of S. mansoni showed 50% positivity for cercarial shedding. Both the experimental and control groups showed 100% survival. The pre-patent period lasted until 39days after exposure to miracidia. Exposed snails that showed cercarial shedding exhibited higher growth rates than either exposed snails that did not demonstrate cercarial shedding or uninfected controls. Exposed snails without cercarial shedding and uninfected controls showed no differences in the reproductive parameters evaluated during the patent period; snails experiencing cercarial shedding showed a reduction in fecundity and fertility. These snails began to lay eggs only after the 50th day post miracidia exposure. The haemolymph glucose levels showed an oscillating pattern that decreased during periods of greater mobilisation of energy by the larvae and was accompanied by a depletion of glycogen in the cephalopodal mass and digestive gland. Histopathological examination at 55days showed that the ovotestis was highly atrophied. There was almost complete disappearance of germ cells, and the supporting stroma formed a nearly empty net. At day 45, the infected digestive gland showed a high cylindrical epithelium with little preserved cytoplasm. The contents of the secretory granules of the albumen gland of infected animals stained with Alcian blue (AB), pH 1.0, indicating the presence of sulphated carbohydrates. Thus, parasitic castration in the B. glabrata-S. mansoni model may be regulated directly and indirectly by the developmental stage of the trematode and the biochemical and histopathological alterations during the patent period of infection.
BibTeX:
 @article{Faro2013, author = {Faro, Marta Julia and Perazzini, Mariana and Corrêa, Lygia Dos Reis and Mello-Silva, Clélia Christina and Pinheiro, Jairo and Mota, Ester Maria and de Souza, Samaly and de Andrade, Zilton and Júnior, Arnaldo Maldonado}, title = {Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni.}, journal = {Exp Parasitol}, year = {2013}, volume = {134}, number = {2}, pages = {228--234}, url = {http://dx.doi.org/10.1016/j.exppara.2013.03.020}, doi = {10.1016/j.exppara.2013.03.020} } 
Guzman-Silva MA, Santos HLC, Peralta RS, Peralta JM and de Macedo HW (2013), "Experimental amoebic liver abscess in hamsters caused by trophozoites of a Brazilian strain of Entamoeba dispar", Experimental Parasitology., May, 2013. Vol. 134(1), pp. 39-47. Academic Press Inc Elsevier Science.
Abstract: It has been claimed that amoebic molecules such as amoebapore, galactose/N-acetyl galactosamine inhibitable lectin, and cysteine proteases are responsible for host tissue destruction and are present in both pathogenic Entamoeba histolytica and non-pathogenic Entamoeba dispar. Some reports have provided evidence that after infection with E. dispar, pathological changes may occur in some humans. The aim of this study was to evaluate E. dispar pathogenicity by comparing it to the pathogenicity of E. histolytica through liver abscesses induced in hamsters. Syrian golden hamsters were challenged by intrahepatic inoculation with the 03C E. dispar strain or with two strains of E. histolytica (HM1:IMSS and EGG) to compare their virulence grades. As control groups, we used bacterial flora and Pavlova's modified medium. Lesions were verified at 1, 3 and 6 days after inoculation. Multiplex Polymerase Chain Reaction was performed to characterize each strain using EdP1/EdP2 and EhP1/EhP2 primers. The EGG and HM1:IMSS E. histolytica strains and 03C E. dispar were able to cause liver lesions. The EGG strain caused extensive hepatic abscesses, and trophozoites were found in the lesions throughout the three periods of study. The HM1:IMSS strain caused smaller abscesses when compared to EGG lesions; however, trophozoites were observed at 1 and 3 days after inoculation. The 03C E. dispar strain caused intermediate abscesses when compared to the others; trophozoites were observed in all periods analyzed. The EGG strain caused progressive evolution of the injury, which differed from the HM1:IMSS and 03C strains. These results strongly suggest that the 03C E. dispar strain is pathogenic in the experimental hamster model. Additional studies are necessary to identify potential factors that regulate the manifestation of virulence of this strain and others. (c) 2013 Elsevier Inc. All rights reserved.
BibTeX:
 @article{Guzman-Silva2013, author = {Guzman-Silva, M. A. and Santos, H. L. C. and Peralta, R. S. and Peralta, J. M. and de Macedo, H. W.}, title = {Experimental amoebic liver abscess in hamsters caused by trophozoites of a Brazilian strain of Entamoeba dispar}, journal = {Experimental Parasitology}, publisher = {Academic Press Inc Elsevier Science}, year = {2013}, volume = {134}, number = {1}, pages = {39--47}, doi = {10.1016/j.exppara.2013.01.015} } 
Santos HLC, Bandyopadhyay K, Bandea R, Peralta RHS, Peralta JM and Da Silva AJ (2013), "LUMINEX (R): a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools", Parasites & Vectors., March, 2013. Vol. 6, pp. 69. Biomed Central Ltd.
Abstract: Background: Six species of the genus Entamoeba, i.e., E. histolytica, E. dispar, E. moshkovskii, E. polecki, E. coli, and E. hartmanii can be found in human stools. Among these, only E. histolytica is considered to be pathogenic, causing intestinal and extra-intestinal disease, but it is morphologically identical to E. dispar and E. moshkovskii. In general, E. polecki, E. coli, and E. hartmanii can be differentiated morphologically from E. histolytica, but some of their diagnostic morphologic features may overlap creating issues for the differential diagnosis. Moreover, the previous inability to differentiate among Entamoeba species has limited epidemiologic information on E histolytica. The objective of this study was to develop a rapid, high-throughput screening method using Luminex technique for the simultaneous detection and differentiation of Entamoeba species. Methods: PCR amplification was performed with biotinylated Entamoeba sp 18S rRNA gene primers, designed to amplify a fragment ranging from 382 to 429 bp of the Entamoeba spp studied. Regions of this fragment that could differentiate among E. histolytica, E. moshkovskii, E. dispar, E. hartmanii and E. coli were selected to design hybridization probes to link to Luminex beads. The assay was standardized with cloned DNA samples of each species and evaluated with 24 DNA extracts from samples obtained from individuals diagnosed with these amebas in their stools. Results: Using this approach we were able to correctly identify E. histoltyica, E. dispar, E hartmanni, E. coli and E. moshkovskii in all specimens studied. From twenty four samples tested by microscopy, PCR/DNA Sequencing and real-time PCR, 100% agreed with PCR-Luminex assay for identification of E. dispar, E. moshkovskii, E. hartmanni, E. histolytica, and E. coli. Conclusion: These results show that this method could be used in the diagnostic detection of Entamoeba spp in fecal samples. This diagnostic test was useful to clearly distinguish E histolytica from other species and also to strengthen epidemiologic data on Entamoeba spp.
BibTeX:
 @article{Santos2013, author = {Santos, H. L. C. and Bandyopadhyay, K. and Bandea, R. and Peralta, R. H. S. and Peralta, J. M. and Da Silva, A. J.}, title = {LUMINEX (R): a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools}, journal = {Parasites & Vectors}, publisher = {Biomed Central Ltd}, year = {2013}, volume = {6}, pages = {69}, doi = {10.1186/1756-3305-6-69} } 

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