Salvatore Giovanni De Simone
Vinculo Institucional:
Quadro/Tesouro
Função: Pesquisador/Chefe de Laboratorio
Unidade: IOC
Departamento: Bioquímica e Biologia Molecular
Laboratório: Bioquímica de Proteinas
Endereço: Av. Brasil 4365
Bairro: Manguinhos
Cidade: Rio de Janeiro
Estado: RJ
CEP: 21045-900
E-mail: dsimone@gene.dbbm.fiocruz.br
Telefone: (21)2907549 R127
Fax: (21)5903495
Maior Titulação: Doutorado
Instituição: UFRJ
Conclusão: 1986
Graduação: Ciências Biológicas
Instituição: UERJ
Conclusão: 1976
Áreas de Atuação: Ciências Básicas
Linhas de Pesquisa
Síntese e Atividade Biológica de Peptídeos e Inibidores
Enzimáticos/Bioquímica Estrutural e Funcional de Proteinas de Agentes
Infecciosos e Parasitários
Equipe
Salvatore Giovanni De Simone (Pesq)
Octavio Antunes (Pesq)
Vera Bongertz (Pesq)
Luciana Jansen Figueiredo (Pesq)
Andre Luis (Pesq)
Felipe Augusto Lima de Oliveira Saraiva (Estudante)
Projeto
Desenvolvimento de substâncias peptidomiméticas: inibidores potenciais
da HIV-1 protease
Período: 1997-1999
Apoio: FIOCRUZ-PAPES A
Principais Produções
Giovanni De Simone S, Torres da Matta J. & Nery da Matta A.
(1994). Acetilcholinesterase and non-specific esterase activities during
the regeneration of planaria Dugesia tigrina (Girard). Z. Naturforschung
49C: 501-507.
Nery da Matta A, Kanaan S, Giovanni De Simone S, Pires ET, Quintana E and
Torres da Matta J. (1994). Lipids concentration during the regeneration of
the planarian Dugesia tigrina (Girard). Comp. Biochem. Physiol. 108A:
543-547.
Giovanni De Simone S, Santos R., Araujo MF & Pinho RT. (1994).
Preparative isolation of the lectin jacalin by anion exchange
high-performance liquid chromatography. J. Chromatography A 688: 357-362.
Silveira IAFB, Jessouroun E & Giovanni De Simone S. (1994). Rapid
method using high performance liquid chromatography for purification of
tetanus toxoid. Mem. Inst. Oswaldo Cruz 89(4): 593-594.
Alves CR, Cortes-Real S, Bourguignon SC, Venyl FBZ, De Freitas-Rosa M,
Corrêa AFS and Giovanni De Simone S. (1995). Identification of
cross-reactive epitopes in papainase and cysteine proteinases from
Leishmania sp. Acta Microscópica 4 : 35
Ferreira-Cruz MF, Giovanni De Simone S, Banic D, Carvalheiro M, Camus D
& Daniel-Ribeiro CT (1996). The use of software as an approach to
predic Tantigenic regions of Plasmodium falciparum peptides. Parasite
Immunol. 18: 159-161.
Giovanni De Simone S, Fagundes SA., Soares ALA & Danelli MG (1996).
Isolation and N-terminal amino acid sequence determination of Class 1-5
outer membrane proteins from a clone of Neisseria meningiditis serogroup B
isolated in Brazil.Mem. Inst. Oswaldo Cruz 91(1): 111-116.
Cabral MMO, Garcia ES, Rembold H., Giovanni De Simone S, Kelecon A. (1966)
Anti-moulting activity in Brazilian Melia azedarach. Mem. Inst. Oswaldo
Cruz 91(1): 117-118.
Aguiar AS, Melgarejo AR & Giovanni De Simone S. (1996) Purification
and partial characterization of a thrombin-like enzyme/giroxin from
Buschmaster (Lachesis muta rhombeata) venom. Toxicon 34(5): 555-565.
Aguiar AS, Melgarejo CR, Alves CR & Giovanni De Simone S (1997).
Purification of crotoxin and crotactine from Crotalus durissus terrificus
venom using a single step on rotofor cell. Braz. J. Med. Biol. Res. 30(1):
25-28.
Bourguignon SC, Alves CR & Giovanni De Simone S (1997) Detrimental
effect of nitric oxide on Trypanosoma Cruzi and Leishmania major like
cells. Acta Tropica 66: 109-118.
Giovanni-De-Simone S, Aguiar AS, Gimenez AR, Novellino K & Moura RS
(1997) Purification, properties, and N-terminal amino acid sequence of a
kininogenin from the venom of Lachesis muta rhombeata (buschmaster). J.
Prot. Chem. 16: 809-817.
Bourguignon S, Pacheco R & Giovanni-De-Simone S (1998) Purification
and partial characterization of Trypanosoma Cruzi triosephosphate
isomerase. Mem. Inst. Oswaldo Cruz 93: 219-224.
Souza ALA, Batoreu NM & Giovanni De Simone S (1998) Single-step
purification of class 5 outer membrane protein from Neisseria meningitidis
using preparative electrophoresis. Biomedical Letters 57: 7-13.
Giovanni De Simone S, Hassón-Voloch A, Batista e Silva C & Nery da
Matta A (1998) Purification and partial characterization of
glyceraldehyde-phosphate dehydrogenase from Electric Organ of
Electrophorus electricus (L.) Z. Naturfosch 53C:416-420.
Alves CR, Giovanni-De-Simone S. Moreno MLV, Finkelstein LC &
Côrtes-Real S (1998) Immunogold labeling of cysteine proteinases in
cutaneous and visceral Leishmania spp. Acta Microscópica 4: 347-348.
Giovanni-De-Simone S, Melo G e Confort EM (1999) Purification and partial
characterization of Leishmania amazonensis glyceraldehydephosphate
dehydrogenase. Biomedical Letters 59(232):121-130
Chaia AA,Giovanni-De-Simone S, Souza MJ, Lima APCA, Branquinha MH &
Vermelho AB (1999) Identification and properties of two extracellular
proteases from Brevundimonas diminuta. Rev. Microbiol.
Petinati SDG, Branquinha MH, Coelho RRR, Vermelho AB &
Giovanni-De-Simone S (1999) Purification and partial characterization of
na extracellular serine-proteinase of Streptomyces cyaneus, isolated from
Brazilian cerrado soil. J. Appl. Microbiol.
Macedo MGR, Freire MGM, Corsini RE, Giovanni De Simone S, Novelo JC e
Marangoni S (1999) Isolation and characterization of a novel plant lectin
from Talisia esculenta seed. Phytochemistry
Morgado-Diaz JÁ, Alves CR, Soares MJ, Corte-Real S e Giovanni-De-Simone S
(1999) Subcellular localization of serine proteinase in Leihsmania
amazonensis promastigotes
Publicações
em Anais de Congresso
Melgarejo AR, Aguiar AS & Giovanni De Simone S (1994).
Electrophoresis analysis of snake venoms and PLA2 using lecitin-containing
polyacrylamide gels.(XXIII Reun. Anual SBBq, Caxambú-MG, M41).
Santos R, Alves CR & Giovanni De Simone S. (1994) Isolation and
partial characterization of 3'nucleotidase from promastigotes of
Leishmania major like (XXIII Reun. Anual SBBq, Caxambú-MG, B49).
Aguiar AS, Melgarejo AR & Giovanni De Simone S (1994). A simple
purification of a serine proteinase from the Lachesis muta rhombeata venom
and N-terminal amino acid sequence determination. (XXIII Reun. Anual SBBq,
Caxambú-MG). M57.
Ferreira da Cruz MF, Giovanni De Simone S, Banic DM, Canto-Carvalheiro M,
Camus D & Daniel-Ribeiro CT (1994). Can the use of software predict
antigenic regions of Plasmodium falciparum peptides? (8th International
Congress of Parasitology, Izmir-Turkey).
Veyl FBZ, Novellino KVA, Alves CR & Giovanni De Simone S. (1994)
Proteinases activities in Leishmania major-like. Mem. Inst. Oswaldo Cruz.,
89, Suppl I, p92 . XXI Reun. An. Pesq. Bras. Doença de Chagas,
Caxambú-MG).
Santos R., Alves CR & Giovanni De Simone S. (1994) 3' Nucleotidase
activity in various species of trypanosomatides. Mem. Inst. Oswaldo Cruz.,
89, Suppl I, p82 (XXI Reun. An. Pesq. Bras. Doença de Chagas, Caxambú-MG).
Bourguignon SC & Giovanni De Simone S. (1994) Partial characterization
of Trypanosoma Cruzi triosephosphate isomerase. Mem. Inst. Oswaldo Cruz.,
89, Suppl I, p81 (XXI Reun. An. Pesq. Bras. Doença de Chagas, Caxambú-MG).
Alves CR, Santos R & Giovanni De Simone S. (1994) Isolation and
partial characterization of a cysteine proteinase from Leishmania
major-like. Mem. Inst. Oswaldo Cruz., 89, Suppl I, p93 (XXI Reun. An. Pesq.
Bras. Doença de Chagas, Caxambú-MG).
Ferreira da Cruz MF, Giovanni De Simone S, Banic DM, Canto-Carvalheiro M,
Camus D & Daniel-Ribeiro CT (1995). Antigenic predictions for
Plasmodium falciparum peptides (International Congress of Immunology, San
Francisco, CA, USA).
Giovanni De Simone S, Aguiar AS, Novellino & Melgarejo A. (1995)
Identification and partial characterization of a kallikrein-like protein
from Lachesis muta rhombeata venom (SBBq).
Alves CR, Giovanni De Simone S, Meirelles MN, Correa AFS and Cortes-Real
S. (1995). Immunocytochemical ultrastructural methods for localization of
cysteine proteinases in two isolates of Leishmania major like using
anti-papain antibody. 3th Interamerican Congress on Electron Microscopy
and XV Meeting of the Braz. Soc. for Electron Microscopy, Caxambú, MG.
Jessouroun E, Bernardo SM, Silveira IAFB, Higa CO, Giovanni De Simone S
and Serodio LR (1995) Purificação do toxoide tetânico: Grau de pureza,
imunogenicidade e reversão. XVIII Cong. Bras. Microbiologia, Santos, SP.
Alves CR, Cortes-Real S, Bourguignon SC, Venyl FBZ, De freitas-Rosa M,
Corrêa AFS and Giovanni De Simone S. (1995). Identification of
cross-reactive epitopes in papainase and cysteine proteinases from
Leishmania sp. Ren. An. Pesq. Bras. Doença de Chagas, Caxambú, MG.
Mendonça-Lima FW, Alves CR, Rosa MF, Giovanni De Simone S and Atta AM
(1996). Digestion of human IgG by Leishmania hagasi metallopeptidase. Mem.
Inst. Oswaldo Cruz 91, Suppl, p163.
Hein RC, Silva JT, Giovanni De Simone S, Panek AD and Paschoalin VMF
(1997) Maltose induced yeast proteins modulated by calmodulin. XII Reun.
Anu. FESBE, Caxambú, MG (10.015).
Hein RC, Silva JT, Giovanni De Simone S, Ferreira JC, Panek AD and
Paschoalin VMF (1997) Identification of Ca++/calmodulin binding proteins
induced by maltose in Saccharomyces cerevisiae. Reun. Anu. SBBq, Caxambú,
MG.
Giovanni De Simone S., Oeistreicher EG, Pinto GF, Gonçalves LPB, Marques
CESS, Sabino SJ, Gomes M, Lobo VS, Iódice B, Ribeiro LP, Figueiredo LJO,
Puzer L, Sales A, Gomes AA and Antunes OAC (1997). Síntese de (Pró)-alfa
e beta-amino ácidos e (Pró)-dipeptídeos: Blocos quirais de construção
para inibidores da HIV-1 e HIV-2 proteases. 2o Simpósio Bras. Pesq. Bás.
em HIV/AIDS, Angra dos Reis, Rio de Janeiro (091).
Giovanni De Simone S, Soares SAF, Barroso DE and Souza ALA. (1997) Linear
epitopes of the variable region 1 (VR1) of the class 1 outer membrane
protein from a Neisseria meningitidis group B (N44/89) strain. XXII Reun.
Anual da SBI.
Soares SAF, Batoreu NM and Giovanni De Simone S (1997) Biochemical and
immunological characterization of a class 5 outer membrane protein from a
Neisseria meningitidis group B strain isolated in Brazil. XXII Reun. Anual
da SBI.
Bourguignon SC, Alves CR and Giovanni De Simone S. (1997). Detrimental
effect of nitric oxide on Trypanosoma Cruzi and Leishmania major-like
cells.
Morgado Díaz JA, Alves CR, Soares MJ and Giovanni De Simone S. (1997).
Preliminar studies on the subcellular localization of acid proterase from
Leishmania amazonensis promastigotes.
Mattos A, Almeida Souza A, Morgado Díaz and Giovanni De Simone S (1997).
Detection of alkaline phosphatase activity on cell surface of promastigote
forms of Leishmania amazonensis.
Lopez RES and Giovanni De Simone S (1997). Purification and partial
characterization of Leishmania amazonensis serine proteases.
Bourguignon SC, Alves CR and Giovanni De Simone S. (1997). Detrimental
effect of nitric oxide on Trypanosoma Cruzi and Leishmania major-like
cells.Bourguignon SC, Meirelles MN, Pacheco R and Giovanni De Simone S
(1997). Purification and partial characterization of Trypanosoma Cruzi
triosephosphate isomerase.
Oliveira ML, Giovanni De Simone S & Beltramini LM (1998) Purification,
chemistry and physic characterization of two lectins of low and high
molecular weight from Caesalpina tinctoria fruits. XXVII Reun. An. SBBq
(M96), Caxambú, MG.
Ferreira FM, Oliveira PSL, Beltramini L., Moreira RA, Giovanni De Simone S
& Oliva G (1998). Initial structural analysis of frutalin, a lectin
from Artocarpus inncisa seeds. XXVII Reun. An. SBBq (M23), Caxambú, MG.
Petinate SDG, Martins RM, Branquinha MH, Coelho RRR, Vermelho AB &
Giovanni De Simone S (1998) Purification and partial characterization of
an extracelular serine protease from Streptomyces cyaneus. XXVII Reun. An.
SBBq (K11), Caxambú, MG..
Figueiredo LJO, Antunes OAC & Giovanni De Simone S (1998) A QSAR study
on HIV-1 protease inhibitors: creating a theorical model for HIV-1
inhibition. XXVII Reun. An. SBBq (M18), Caxambú, MG
Atividades Docentes &
Serviços
"II e III Curso Teórico-Prático de Purificação de
Proteínas" do Curso de Pós-graduação em Biologia Celular e
Molecular, FIOCRUZ-BCM 075, (5-6/92;3/93;5/1994).
Disciplina de Biologia Molecular para o programa de Pós-graduação em
Biologia Parasitária e Biologia Celular e Molecular, FIOCRUZ (94/96).
Disciplina de "Sequenciamento de Proteínas" do Curso de
Pós-graduação em Biologia Celular e Molecular, FIOCRUZ-BCM , (11/1994).
Disciplina do "IV-VIII Curso Teórico-Prático de Purificação e
Sequenciamento de Proteínas" do Curso de Pós-graduação em
Biologia Celular e Molecular, FIOCRUZ-BCM , (1995-1999).
Disciplina de Fracionamento Celular em Modelos Parasitários do Curso de
Pós-graduação em Biologia Celular e Molecular, FIOCRUZ-BCM (07/1997).
Princípios bioquímicos para determinação de epitopos de linfícitos T
e B em modelos de antígenos parasitários do Curso de Pós-graduação em
Biologia Celular e Molecular, FIOCRUZ-BCM (1997/1999).
Disciplina de Modelagem Molecular, Curso de Pós-graduação em Biologia
Celular e Molecular, FIOCRUZ-BCM (1999).
Áreas Potenciais de Treinamento
Bioquímica de Proteínas
Síntese de Peptídeos
Modelagem Molecular
Imunologia Molecular