Área de PDI em Pesquisa, Desenvolvimento e Inovação em Doenças Virais e Rickettsioses 2013
Veja, abaixo, a relação de artigos científicos publicados pelo IOC, na referida Área Temática, organizados em ordem alfabética crescente:
| Amaral SS, Oliveira AG, Marques PE, Quintao JLD, Pires DA, Resende RR, Sousa BR, Melgaco JG, Pinto MA, Russo RC, Gomes AKC, Andrade LM, Zanin RF, Pereira RVS, Bonorino C, Soriani FM, Lima CX, Cara DC, Teixeira MM, Leite MF and Menezes GB (2013), "Altered responsiveness to extracellular ATP enhances acetaminophen hepatotoxicity", Cell Communication and Signaling., February, 2013. Vol. 11, pp. 10. Biomed Central Ltd. [DOI] |
| Abstract: Background: Adenosine triphosphate (ATP) is secreted from hepatocytes under physiological conditions and plays an important role in liver biology through the activation of P2 receptors. Conversely, higher extracellular ATP concentrations, as observed during necrosis, trigger inflammatory responses that contribute to the progression of liver injury. Impaired calcium (Ca2+) homeostasis is a hallmark of acetaminophen (APAP)-induced hepatotoxicity, and since ATP induces mobilization of the intracellular Ca2+ stocks, we evaluated if the release of ATP during APAP-induced necrosis could directly contribute to hepatocyte death. Results: APAP overdose resulted in liver necrosis, massive neutrophil infiltration and large non-perfused areas, as well as remote lung inflammation. In the liver, these effects were significantly abrogated after ATP metabolism by apyrase or P2X receptors blockage, but none of the treatments prevented remote lung inflammation, suggesting a confined local contribution of purinergic signaling into liver environment. In vitro, APAP administration to primary mouse hepatocytes and also HepG2 cells caused cell death in a dose-dependent manner. Interestingly, exposure of HepG2 cells to APAP elicited significant release of ATP to the supernatant in levels that were high enough to promote direct cytotoxicity to healthy primary hepatocytes or HepG2 cells. In agreement to our in vivo results, apyrase treatment or blockage of P2 receptors reduced APAP cytotoxicity. Likewise, ATP exposure caused significant higher intracellular Ca2+ signal in APAP-treated primary hepatocytes, which was reproduced in HepG2 cells. Quantitative real time PCR showed that APAP-challenged HepG2 cells expressed higher levels of several purinergic receptors, which may explain the hypersensitivity to extracellular ATP. This phenotype was confirmed in humans analyzing liver biopsies from patients diagnosed with acute hepatic failure. Conclusion: We suggest that under pathological conditions, ATP may act not only an immune system activator, but also as a paracrine direct cytotoxic DAMP through the dysregulation of Ca2+ homeostasis. |
BibTeX: @article{Amaral2013, author = {Amaral, S. S. and Oliveira, A. G. and Marques, P. E. and Quintao, J. L. D. and Pires, D. A. and Resende, R. R. and Sousa, B. R. and Melgaco, J. G. and Pinto, M. A. and Russo, R. C. and Gomes, A. K. C. and Andrade, L. M. and Zanin, R. F. and Pereira, R. V. S. and Bonorino, C. and Soriani, F. M. and Lima, C. X. and Cara, D. C. and Teixeira, M. M. and Leite, M. F. and Menezes, G. B.}, title = {Altered responsiveness to extracellular ATP enhances acetaminophen hepatotoxicity}, journal = {Cell Communication and Signaling}, publisher = {Biomed Central Ltd}, year = {2013}, volume = {11}, pages = {10}, doi = {10.1186/1478-811X-11-10} } |
| Brandão CPU, Marques BLC, Marques VA, Villela-Nogueira CA, Do Ó KMR, de Paula MT, Lewis-Ximenez LL, Lampe E, Sá Ferreira JA and Villar LM (2013), "Simultaneous detection of hepatitis c virus antigen and antibodies in dried blood spots.", J Clin Virol., Jun, 2013. Vol. 57(2), pp. 98-102. [DOI] |
| Abstract: Enzyme immunoassays (EIA) designed to detect hepatitis C virus (HCV) core antigen and anti-HCV antibodies (HCV AgAb) simultaneously can improve the early detection of HCV infection when molecular diagnostic methods are not widely available.To evaluate the suitability of dried blood spot (DBS) samples for detecting HCV AgAb using commercial EIAs.Paired serum and DBS samples were assayed using two commercial EIAs for HCV AgAb (Monolisa™ HCV AgAb ULTRA and Murex HCV AgAb). Manufacturer's recommendations were followed for sera while sample volume, incubation time and cut-off (CO) determination were evaluated for the DBS samples. The values of sensitivity, specificity, inter-rater agreement, detection limit, assay precision and stability of DBS samples at different conditions (22-26°C, 2-8°C and -20°C) were determined.It was necessary to increase the DBS sample volume fourfold compared to the sera samples to approximate the DBS Optical Density (OD) values to the sera OD values. Using ROC curve to recalculate CO values for the DBS samples, sensitivity was 97.5% for both EIAs, while the specificity was 99.71% for Monolisa™ HCV AgAb ULTRA and 95.95% for Murex HCV AgAb. Accurate testing results were obtained with DBS samples for 60 days at all conditions evaluated; storage at -20°C resulted in low OD variation. Both EIAs demonstrated the same limit of detection among DBS samples [estimated viral load of 3.1 International Units per millilitre (IU/mL)] and low OD value variability in repetitivity and reproducibility studies.DBS samples can be used for the detection of HCV AgAb by EIA as they present comparable performance characteristics and excellent stability among various storage conditions. |
BibTeX: @article{Brandao2013, author = {Brandão, C P U. and Marques, B L C. and Marques, V. A. and Villela-Nogueira, C. A. and Do Ó, K M R. and de Paula, M. T. and Lewis-Ximenez, L. L. and Lampe, E. and Sá Ferreira, J. A. and Villar, L. M.}, title = {Simultaneous detection of hepatitis c virus antigen and antibodies in dried blood spots.}, journal = {J Clin Virol}, year = {2013}, volume = {57}, number = {2}, pages = {98--102}, url = {http://dx.doi.org/10.1016/j.jcv.2013.02.014}, doi = {10.1016/j.jcv.2013.02.014} } |
| Calgua B, Fumian T, Rusinol M, Rodriguez-Manzano J, Mbayed VA, Bofill-Mas S, Miagostovich M and Girones R (2013), "Detection and quantification of classic and emerging viruses by skimmed-milk flocculation and PCR in river water from two geographical areas", Water Research., May, 2013. Vol. 47(8), pp. 2797-2810. Pergamon-elsevier Science Ltd. [DOI] |
| Abstract: Molecular techniques and virus concentration methods have shown that previously unknown viruses are shed by humans and animals, and may be transmitted by sewage-contaminated water. In the present study, 10-L river-water samples from urban areas in Barcelona, Spain and Rio Janeiro, Brazil, have been analyzed to evaluate the viral dissemination of human viruses, validating also a low-cost concentration method for virus quantification in fresh water. Three viral groups were analyzed: (i) recently reported viruses, klassevirus (KV), asfarvirus-like virus (ASFLV), and the polyomaviruses Merkel cell (MCPyV), KI (KIPyV) and WU (WUPyV); (ii) the gastroenteritis agents noroviruses (NoV) and rotaviruses (RV); and (iii) the human fecal viral indicators in water, human adenoviruses (HAdV) and JC polyomaviruses (JCPyV). Virus detection was based on nested and quantitative PCR assays. For KV and ASFLV, nested PCR assays were developed for the present study. The method applied for virus concentration in fresh water samples is a one-step procedure based on a skimmed-milk flocculation procedure described previously for seawater. Using spiked river water samples, inter- and intra-laboratory assays showed a viral recovery rate of about 50% (20-95%) for HAdV, JCPyV, NoV and RV with a coefficient of variation <= 50%. HAdV and JCPyV were detected in 100% (12/12) of the river samples from Barcelona and Rio de Janeiro. Moreover, NoV GGII was detected in 83% (5/6) and MCPyV in 50% (3/6) of the samples from Barcelona, whereas none of the other viruses tested were detected. NoV GGII was detected in 33% (2/6), KV in 33% (2/6), ASFLV in 17% (1/6) and MCPyV in 50% (3/6) of the samples from Rio de Janeiro, whereas KIPyV and WUPyV were not detected. RV were only analyzed in Rio de Janeiro and resulted positive in 67% (4/6) of the samples. The procedure applied here to river water represents a useful, straightforward and cost-effective method that could be applied in routine water quality testing. The results of the assays expand our understanding of the global distribution of the viral pathogens studied here and their persistence in the environment. (C) 2013 Elsevier Ltd. All rights reserved. |
BibTeX: @article{Calgua2013, author = {Calgua, B. and Fumian, T. and Rusinol, M. and Rodriguez-Manzano, J. and Mbayed, V. A. and Bofill-Mas, S. and Miagostovich, M. and Girones, R.}, title = {Detection and quantification of classic and emerging viruses by skimmed-milk flocculation and PCR in river water from two geographical areas}, journal = {Water Research}, publisher = {Pergamon-elsevier Science Ltd}, year = {2013}, volume = {47}, number = {8}, pages = {2797--2810}, doi = {10.1016/j.watres.2013.02.043} } |
| Castro TX, Cubel Garcia RCN, Costa EM, Leal RM, Xavier MdPT and Leite JPG (2013), "Molecular characterisation of calicivirus and astrovirus in puppies with enteritis.", Vet Rec., May, 2013. Vol. 172(21), pp. 557. [DOI] |
BibTeX: @article{Castro2013, author = {Castro, T. X. and Cubel Garcia, R C N. and Costa, E. M. and Leal, R. M. and Xavier, M da P T. and Leite, J P G.}, title = {Molecular characterisation of calicivirus and astrovirus in puppies with enteritis.}, journal = {Vet Rec}, year = {2013}, volume = {172}, number = {21}, pages = {557}, url = {http://dx.doi.org/10.1136/vr.101566}, doi = {10.1136/vr.101566} } |
| Da Silva MFM, Gómez MM, Rose TL, Volotão EdM, Carvalho-Costa FA, Bello G and Leite JPG (2013), "VP8(∗)P[8] lineages of group A rotaviruses circulating over 20years in Brazil: Proposal of six different sub-lineages for P[8]-3 clade.", Infect Genet Evol., Jun, 2013. Vol. 16C, pp. 200-205. [DOI] |
| Abstract: Group A rotaviruses (RVA) is the most important cause of severe gastroenteritis among children worldwide. Vaccination is considered the best alternative among public health measures to reduce and prevent the global burden caused by RVA infections. Rotarix™, a monovalent vaccine based on a human strain with a G1P[8]-1 specificity, was introduced in the National Brazilian Immunization Programs (NIP) in March, 2006. RVA P[8] is the most prevalent P genotype worldwide and four distinct phylogenetic lineages: P[8]-1, -2, -3, and -4 have been described. In the current study phylogenetic analysis of the VP8(∗) gene of 135 RVA P[8] Brazilian strains, in combination with G1, G3, G5 or G9 VP7 genotype, collected from 1986 to 2011 were carried out for a better understanding of the evolution of this viral genotype in Brazil. Lineages P[8]-1, P[8]-2, and P[8]-3 were observed circulating in Brazil. In 2001 these three P[8] lineages co-circulated simultaneously and this is the first report in South America to date. Considering the P[8] lineage and the G genotype, all G3 strains were related to lineage P[8]-3, whereas the G9 strains were related to P[8]-2 and P[8]-3 and G1 and G5 were related to P[8]-1, P[8]-2, and P[8]-3. In addition, the phylogenetic analysis based on estimate of genetic distances between P[8]-3 strains and the definition of a 1.5% cutoff value (with relevant statistical support) it was possible to propose a new classification for the P[8]-3 lineage into six different sub-lineages: P[8]-3.1 to P[8]-3.6. These findings reinforce the notion of the existence of constraints within specific RVA strains populations. The results obtained in this study reinforce the importance of a continuous RVA surveillance of circulating strains in order to predict the possible variants that will circulate in a country, assess the effects of vaccination on RVA circulating strains, and ultimately help in the design, challenges, and prospects of RVA vaccines. |
BibTeX: @article{daSilva2013, author = {Da Silva, Marcelle Figueira Marques and Gómez, Mariela Martínez and Rose, Tatiana Lundgren and Volotão, Eduardo de Mello and Carvalho-Costa, Filipe Anibal and Bello, Gonzalo and Leite, Jose Paulo Gagliardi}, title = {VP8(∗)P[8] lineages of group A rotaviruses circulating over 20years in Brazil: Proposal of six different sub-lineages for P[8]-3 clade.}, journal = {Infect Genet Evol}, year = {2013}, volume = {16C}, pages = {200--205}, url = {http://dx.doi.org/10.1016/j.meegid.2013.01.004}, doi = {10.1016/j.meegid.2013.01.004} } |
| de Carvalho LG, Marchevsky RS, Dos Santos DR, de Oliveira JM, de Paula VS, Lopes LM, Van der Poel WH, González JE, Munné MS, Moran J, Cajaraville AC, Pelajo-Machado M, Cruz OG and Pinto MA (2013), "Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis.", BMC Infect Dis., Oct, 2013. Vol. 13(1), pp. 495. [DOI] |
| Abstract: Hepatitis E virus (HEV) has been described as an emerging pathogen in Brazil and seems to be widely disseminated among swine herds. An autochthonous human case of acute hepatitis E was recently reported. To obtain a better understanding of the phenotypic profiles of both human and swine HEV strains, a experimental study was conducted using the animal model, Macaca fascicularis.Six cynomolgus monkeys (Macaca fascicularis) were inoculated intravenously with swine HEV genotype 3 that was isolated from naturally and experimentally infected pigs in Brazil and the Netherlands. Two other monkeys were inoculated with HEV genotype 3 that was recovered from Brazilian and Argentinean patients with locally acquired acute and fulminant hepatitis E. The haematological, biochemical, and virological parameters of all animals were monitored for 67 days.Subclinical hepatitis was observed in all monkeys after inoculation with HEV genotype 3 that was recovered from the infected swine and human patients. HEV RNA was detected in the serum and/or faeces of 6 out of the 8 cynomolgus monkeys between 5 and 53 days after inoculation. The mild inflammation of liver tissues and elevations of discrete liver enzymes were observed. Seroconversions to anti-HEV IgM and/or IgG were detected in 7 animals. Reactivities to anti-HEV IgA were also detected in the salivary samples of 3 animals. Interestingly, all of the infected monkeys showed severe lymphopenia and a trend toward monocytosis, which coincided with elevations in alanine aminotransferase and antibody titres.The ability of HEV to cross the species barrier was confirmed for both the swine (Brazilian and Dutch) and human (Argentinean) strains, thus reinforcing the zoonotic risk of hepatitis E in South America. Cynomolgus monkeys that were infected with HEV genotype 3 developed subclinical hepatitis that was associated with haematological changes. Haematological approaches should be considered in future studies of HEV infection. |
BibTeX: @article{deCarvalho2013, author = {de Carvalho, Lilian G. and Marchevsky, Renato S. and Dos Santos, Debora Rl and de Oliveira, Jaqueline M. and de Paula, Vanessa S. and Lopes, Leilane M. and Van der Poel, Wilhelmus Hm and González, Jorge E. and Munné, Maria S. and Moran, Julio and Cajaraville, Ana Carolina and Pelajo-Machado, Marcelo and Cruz, Oswaldo G. and Pinto, Marcelo A.}, title = {Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis.}, journal = {BMC Infect Dis}, year = {2013}, volume = {13}, number = {1}, pages = {495}, url = {http://dx.doi.org/10.1186/1471-2334-13-495}, doi = {10.1186/1471-2334-13-495} } |
| Delvaux N, de Paula VS, Espirito-Santo MP, da Silva EF, Miguel JC, de Oliveira JC, da Silva AS, Vieira YR, Lampe E and Villar LM (2013), "Knowledge about viral hepatitis among participants of Gay Pride Event in Brazil", Brazilian Journal of Infectious Diseases., May, 2013. Vol. 17(3), pp. 377-378. Contexto. [DOI] |
BibTeX: @article{Delvaux2013a, author = {Delvaux, N. and de Paula, V. S. and Espirito-Santo, M. P. and da Silva, E. F. and Miguel, J. C. and de Oliveira, J. C. and da Silva, A. S. and Vieira, Y. R. and Lampe, E. and Villar, L. M.}, title = {Knowledge about viral hepatitis among participants of Gay Pride Event in Brazil}, journal = {Brazilian Journal of Infectious Diseases}, publisher = {Contexto}, year = {2013}, volume = {17}, number = {3}, pages = {377--378}, doi = {10.1016/j.bjid.2012.09.015} } |
| Delvaux N, Paula VSd, Espírito-Santo MPd, Silva EFd, Miguel JC, Oliveira JCd, Silva ASd, Vieira YR, Lampe E and Villar LM (2013), "Knowledge about viral hepatitis among participants of Gay Pride Event in Brazil.", Braz J Infect Dis. Vol. 17(3), pp. 377-378. [DOI] |
BibTeX: @article{Delvaux2013, author = {Delvaux, Nathália and Paula, Vanessa S de and Espírito-Santo, Márcia P do and Silva, Elisangela F da and Miguel, Juliana C. and Oliveira, Jaqueline C de and Silva, Alexandre S da and Vieira, Yasmine R. and Lampe, Elisabeth and Villar, Livia M.}, title = {Knowledge about viral hepatitis among participants of Gay Pride Event in Brazil.}, journal = {Braz J Infect Dis}, year = {2013}, volume = {17}, number = {3}, pages = {377--378}, url = {http://dx.doi.org/10.1016/j.bjid.2012.09.015}, doi = {10.1016/j.bjid.2012.09.015} } |
| De Oliveira RC, Padula PJ, Gomes R, Martinez VP, Bellomo C, Bonvicino CR, Lima DIFE, Bragagnolo C, Caldas ACS, D'Andrea PS and de Lemos ERS (), "Genetic Characterization of Hantaviruses Associated with Sigmodontine Rodents in an Endemic Area for Hantavirus Pulmonary Syndrome in Southern Brazil", Vector-borne and Zoonotic Diseases. [DOI] |
| Abstract: An ecological assessment of reservoir species was conducted in a rural area (Jabora) in the mid-west of the state of Santa Catarina in southern Brazil, where hantavirus pulmonary syndrome is endemic, to evaluate the prevalence of hantavirus infection in wild rodents. Blood and tissue samples were collected from 507 rodents during seven field trips from March 2004 to April 2006. Some of the animals were karyotyped to confirm morphological identification. Phylogenetic reconstructions of rodent specimens, based on the mitochondrial DNA cytochrome b gene sequences, were also obtained. Hantavirus antibody was found in 22 (4.3%) of the 507 rodents: 5 Akodon montensis, 2 Akodon paranaensis, 14 Oligoryzomys nigripes, and 1 Sooretamys angouya. Viral RNAs detected in O. nigripes and A. montensis were amplified and sequenced. O. nigripes virus genome was 97.5% (nt) and 98.4% (nt) identical to sequences published for Araucaria (Juquitiba-like) virus based on N and G2 fragment sequences. Viral sequences from A. montensis strain showed 89% and 88% nucleotide identities in a 905-nt fragment of the nucleocapsid (N) protein-coding region of the S segment when it was compared with two other Akodontine rodent-associated viruses from Paraguay, A. montensis and Akodon cursor, respectively. Phylogenetic analysis showed the cocirculation of two genetic hantavirus lineages in the state of Santa Catarina, one from O. nigripes and the other from A. montensis, previously characterized in Brazil and Paraguay, respectively. The hantavirus associated with A. montensis, designed Jabora virus, represents a distinct phylogenetic lineage among the Brazilian hantaviruses. |
BibTeX: @article{Oliveira, author = {De Oliveira, R. C. and Padula, P. J. and Gomes, R. and Martinez, V. P. and Bellomo, C. and Bonvicino, C. R. and Lima, D. I. F. E. and Bragagnolo, C. and Caldas, A. C. S. and D'Andrea, P. S. and de Lemos, E. R. S.}, title = {Genetic Characterization of Hantaviruses Associated with Sigmodontine Rodents in an Endemic Area for Hantavirus Pulmonary Syndrome in Southern Brazil}, journal = {Vector-borne and Zoonotic Diseases}, doi = {10.1089/vbz.2010.0001} } |
| De Paula Scalioni L, Cruz HM, de Paula VS, Corrêia Oliveira J, Tourinho Dos Santos R, Motta-Castro ARC, Murat PG, Villela-Nogueira CA, Lewis-Ximenez LL, Lampe E and Villar LM (2013), "Importance of collection methods and stability of oral fluid samples for hepatitis B surface antigen detection.", J Clin Lab Anal., May, 2013. Vol. 27(3), pp. 186-194. [DOI] |
| Abstract: Oral fluid (OF) sample collection and stability for HBsAg detection are not fully established. This study aims to investigate the applicability of OF collectors and sample stability for Hepatitis B virus surface antigen detection.Paired serum and OF samples were obtained from 191 individuals, and Chembio (Chembio Diagnostic System, USA) and Salivette (Sarstedt, Germany) devices were used for OF collection. Two HBsAg enzyme immunoassays (EIAs) were used (HBsAg One kit, Radim, Rome, Italy and ETI-MAK-4, DiaSorin, Vercelli, Italy) to determine the most efficient method according OF collector. Sample volume, incubation time, and cutoff (CO) value were evaluated. The stability of OF samples was determined under different environmental conditions.Chembio samples analyzed using DiaSorin EIA without modification of the manufacturer's instructions, demonstrated a sensitivity of 95.24% and a specificity of 100 Salivette samples analyzed with Radim EIA with receiver operating characteristic (ROC) curve for calculating the CO showed a sensitivity of 78.26% and a specificity of 89.88 HBsAg was detected in Chembio and Salivette samples under different environmental conditions, but the Chembio samples were the most stable.Both collectors can be used for HBsAg detection in OF samples, but some modifications of commercial EIAs should be incorporated for Salivette device. OF samples were reliably stable and could be stored for up to 90 days at 2-8°C. |
BibTeX: @article{dePaulaScalioni2013, author = {De Paula Scalioni, Letícia and Cruz, Helena Medina and de Paula, Vanessa Salete and Corrêia Oliveira, Jaqueline and Tourinho Dos Santos, Renata and Motta-Castro, Ana Rita Coimbra and Murat, Paula Guerra and Villela-Nogueira, Cristiane Alves and Lewis-Ximenez, Lia Laura and Lampe, Elisabeth and Villar, Livia Melo}, title = {Importance of collection methods and stability of oral fluid samples for hepatitis B surface antigen detection.}, journal = {J Clin Lab Anal}, year = {2013}, volume = {27}, number = {3}, pages = {186--194}, url = {http://dx.doi.org/10.1002/jcla.21582}, doi = {10.1002/jcla.21582} } |
| Echevarria JM, Gonzalez JE, Lewis-Ximenez LL, dos Santos DRL, Munne MS, Pinto MA, Pujol FH and Rodriguez-Lay LA (2013), "Hepatitis E virus infection in Latin America: A review", Journal of Medical Virology., June, 2013. Vol. 85(6), pp. 1037-1045. Wiley-blackwell. [DOI] |
| Abstract: Data reported during recent years reveal the complex picture of the epidemiology of hepatitis E virus (HEV) infection in Latin America. Whereas in countries like Argentina and Brazil is almost identical to the characteristic of most countries from North America and Europe, HEV in the Caribbean and Mexico involves the water-borne, non-zoonotic viral genotypes responsible for epidemics in Asia and Africa. Nevertheless, Latin America has been considered a highly endemic region for hepatitis E in the scientific literature, a generalization that ignores the above complexity. In addition, reports from isolated Amerindian communities, which display well known, important and very specific epidemiological features for hepatitis B and D virus infections are neither taken into account when considering the epidemiology of hepatitis E in the region. This review updates compilation of the available information for the HEV infection, both among humans and other mammals, in Latin America, discusses the strengths and the weaknesses of our current knowledge, and identifies future areas of research. J. Med. Virol. 85: 10371045, 2013. (c) 2013 Wiley Periodicals, Inc. |
BibTeX: @article{Echevarria2013, author = {Echevarria, J. M. and Gonzalez, J. E. and Lewis-Ximenez, L. L. and dos Santos, D. R. L. and Munne, M. S. and Pinto, M. A. and Pujol, F. H. and Rodriguez-Lay, L. A.}, title = {Hepatitis E virus infection in Latin America: A review}, journal = {Journal of Medical Virology}, publisher = {Wiley-blackwell}, year = {2013}, volume = {85}, number = {6}, pages = {1037--1045}, doi = {10.1002/jmv.23526} } |
| Esona MD, Mijatovic-Rustempasic S, Foytich K, Roy S, Banyai K, Armah GE, Steele AD, Volotão EM, Gomez MM, Silva MFM, Gautam R, Quaye O, Tam KI, Forbi JC, Seheri M, Page N, Nyangao J, Ndze VN, Aminu M, Bowen MD and Gentsch JR (2013), "Human G9P[8] rotavirus strains circulating in Cameroon, 1999-2000: Genetic relationships with other G9 strains and detection of a new G9 subtype.", Infect Genet Evol., Aug, 2013. Vol. 18, pp. 315-324. [DOI] |
| Abstract: Group A rotaviruses (RV-A) are the leading cause of viral gastroenteritis in children worldwide and genotype G9P[8] is one of the five most common genotypes detected in humans. In order to gain insight into the degree of genetic variability of G9P[8] strains circulating in Cameroon, stool samples were collected during the 1999-2000 rotavirus season in two different geographic regions in Cameroon (Southwest and Western Regions). By RT-PCR, 15 G9P[8] strains (15/89=16.8 were identified whose genomic configurations was subsequently determined by complete or partial gene sequencing. In general, all Cameroonian G9 strains clustered into current globally-spread sublineages of the VP7 gene and displayed 86.6-100% nucleotide identity amongst themselves and 81.2-99.5% nucleotide identity with global G9 strains. The full genome classification of all Cameroonian strains was G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 but phylogenetic analysis of each gene revealed that the strains were spread across 4 or more distinct lineages. An unusual strain, RVA/Human-wt/CMR/6788/1999/G9P[8], which shared the genomic constellation of other Cameroonian G9P[8] strains, contained a novel G9 subtype which diverged significantly (18.8% nucleotide and 19% amino acid distance) from previously described G9 strains. Nucleotide and amino acid alignments revealed that the 3' end of this gene is highly divergent from other G9 VP7 genes suggesting that it arose through extensive accumulation of point mutations. The results of this study demonstrate that diverse G9 strains circulated in Cameroon during 1999-2000. |
BibTeX: @article{Esona2013, author = {Esona, M. D. and Mijatovic-Rustempasic, S. and Foytich, K. and Roy, S. and Banyai, K. and Armah, G. E. and Steele, A. D. and Volotão, E. M. and Gomez, M. M. and Silva, M F M. and Gautam, R. and Quaye, O. and Tam, K. I. and Forbi, J. C. and Seheri, M. and Page, N. and Nyangao, J. and Ndze, V. N. and Aminu, M. and Bowen, M. D. and Gentsch, J. R.}, title = {Human G9P[8] rotavirus strains circulating in Cameroon, 1999-2000: Genetic relationships with other G9 strains and detection of a new G9 subtype.}, journal = {Infect Genet Evol}, year = {2013}, volume = {18}, pages = {315--324}, url = {http://dx.doi.org/10.1016/j.meegid.2013.06.005}, doi = {10.1016/j.meegid.2013.06.005} } |
| Espírito-Santo MPd, Brandão-Mello CE, Marques VA, Lampe E and Almeida AJD (2013), "Analysis of hepatitis C virus (HCV) RNA load in platelets of HCV-monoinfected patients receiving antiviral therapy.", Ann Hepatol. Vol. 12(3), pp. 373-379. |
| Abstract: Detection of hepatitis C virus (HCV) has been reported in extrahepatic sites such as peripheral blood mononuclear cells and platelets. Quantitation of HCV-RNA in platelets from patients under antiviral therapy has not been reported.HCV-RNA levels in paired serum and platelet samples of 17 chronically HCV-infected patients were determined at baseline, week 12, end-of-treatment, and 24 weeks after completion of treatment with pegylated interferon plus ribavirin. Quantitation of HCVRNA load was performed using COBAS® TaqMan® HCV Test v 2.0 (lower limit of detection, 25 IU/mL). The cohort predominantly consisted of female (59 with a mean age of 50.7 ± 10.0 years.Measurements of HCV-RNA in relation to different timepoints of therapy revealed baseline viral load was most frequently detected in higher levels in serum than in platelets (5.6 x 104 IU/mL vs. 379.0 IU/mL; p = 0.0002), a trend also demonstrated in most samples throughout the study. HCV-RNA was also found at low levels (< 25.0-314.0 UI/mL) persistently in platelets of three patients who have lost detectable HCV-RNA in serum during antiviral therapy, resulting in virological relapse.HCV-RNA levels are most frequently detected in higher levels in serum than in platelets, independent of timepoint of antiviral therapy. Further studies with an increase in size of the samples are needed to better evaluate whether or not patients who presented HCV-RNA at low levels in platelets after having lost detectable HCV-RNA in serum during antiviral therapy are at increased risk of relapse of HCV infection during follow-up evaluation. |
BibTeX: @article{Espirito-Santo2013, author = {Espírito-Santo, Márcia Paschoal do and Brandão-Mello, Carlos Eduardo and Marques, Vanessa Alves and Lampe, Elisabeth and Almeida, Adilson José De}, title = {Analysis of hepatitis C virus (HCV) RNA load in platelets of HCV-monoinfected patients receiving antiviral therapy.}, journal = {Ann Hepatol}, year = {2013}, volume = {12}, number = {3}, pages = {373--379} } |
| Fumian TM, Vieira CB, Leite JPG and Miagostovich MP (2013), "Assessment of burden of virus agents in an urban sewage treatment plant in Rio de Janeiro, Brazil.", J Water Health., Mar, 2013. Vol. 11(1), pp. 110-119. [DOI] |
| Abstract: Sewage discharge is considered to be the main source of virus contamination in aquatic environments. There is no correlation between the presence of viruses and the presence of fecal coliforms in water; therefore virological markers are needed when monitoring contamination. This study investigates DNA and RNA virus concentrations in wastewater and evaluates a potential virus marker of human contamination. Influent and effluent samples were collected twice a month throughout a 1-year period. Viruses were detected using quantitative polymerase chain reaction protocols; nucleotide sequencing was carried out for virus genotyping. Human adenovirus (HAdV) and polyomavirus JC (JCPyV) were the most prevalent viruses found in influent samples (100 with a virus load that ranged from 10(6) to 10(5) genome copies per liter (gc l(-1)). Norovirus genogroup II (NoV GII) and human astrovirus (HAstV) were less prevalent, and ranged from 10(4) to 10(3)gc l(-1). Quantitative data on virus profiles in wastewaters stress the high level of rotavirus species A environmental dissemination and address the potential of HAdV as a useful virological marker of virus contamination in aquatic environments. This study corroborates other studies performed in developed countries on DNA viruses as good markers of human fecal contamination. |
BibTeX: @article{Fumian2013, author = {Fumian, Tulio Machado and Vieira, Carmen Baur and Leite, José Paulo Gagliardi and Miagostovich, Marize Pereira}, title = {Assessment of burden of virus agents in an urban sewage treatment plant in Rio de Janeiro, Brazil.}, journal = {J Water Health}, year = {2013}, volume = {11}, number = {1}, pages = {110--119}, url = {http://dx.doi.org/10.2166/wh.2012.123}, doi = {10.2166/wh.2012.123} } |
| Galeano ME, Martinez M, Amarilla AA, Russomando G, Miagostovich MP, Parra GI and Leite JP (2013), "Molecular epidemiology of norovirus strains in Paraguayan children during 2004-2005: description of a possible new GII.4 cluster.", J Clin Virol., Oct, 2013. Vol. 58(2), pp. 378-384. [DOI] |
| Abstract: Noroviruses (NoV) have been shown to be an important cause of morbidity and mortality in children worldwide, only second after Group A rotaviruses (RVA). In Paraguay, acute gastroenteritis (AGE) is the third cause of mortality in children ≤5 years old.To analyze the presence and diversity of NoV in Paraguayan children ≤5 years old presenting AGE.Three hundred seventy eight fecal samples, negative for pathogenic bacteria and RVA, were collected from children admitted as ambulatory and hospitalized patients in a large private hospital from Asuncion, Paraguay from 2004 to 2005. The presence and diversity of NoV was determined by two different RT-PCR strategies and nucleotide sequencing.One hundred and sixty one samples were positive for NoV by partial amplification of the viral polymerase gene (RdRp). No seasonality or differences in the viral prevalence for the different age-groups were detected. GII and GI NoVs were associated to 58% and 42% of the infections, respectively. The genotype was determined in 18% (29/161) NoV-positive samples. The genotypes detected were: GII.4 (18, GII.17 (18, GII.6 (14, GII.7 (14, GII.3 (10, GII.5 (3, GII.8 (3, GII.16 (3, GI.3 (14 and GI.8 (3. Amplification of the ORF2 from the GII.4 strains showed the presence of a new GII.4 variant.The results showed a continuous circulation of NoV in children throughout the two years of study and an extensive diversity of genotypes co-circulating, highlighting the need for better surveillance of NoV in Paraguayan children. |
BibTeX: @article{Galeano2013, author = {Galeano, Maria Eugenia and Martinez, Magaly and Amarilla, Alberto A. and Russomando, Graciela and Miagostovich, Marize Pereira and Parra, Gabriel I. and Leite, José Paulo}, title = {Molecular epidemiology of norovirus strains in Paraguayan children during 2004-2005: description of a possible new GII.4 cluster.}, journal = {J Clin Virol}, year = {2013}, volume = {58}, number = {2}, pages = {378--384}, url = {http://dx.doi.org/10.1016/j.jcv.2013.07.008}, doi = {10.1016/j.jcv.2013.07.008} } |
| Gómez MM, da Silva MFM, Zeller M, Heylen E, Matthijnssens J, Ichihara MYT, Rose TL, de Mello Volotão E and Leite JPG (2013), "Phylogenetic analysis of G1P[6] group A rotavirus strains detected in Northeast Brazilian children fully vaccinated with Rotarix™.", Infect Genet Evol., Oct, 2013. Vol. 19, pp. 395-402. [DOI] |
| Abstract: In 2009 the World Health Organization recommended the use of group A rotavirus (RVA) vaccines in all national immunization programs (NIPs) in order to control severe RVA gastroenteritis disease. In Brazil, Rotarix™ was introduced in the NIP in March 2006, and a significant reduction in mortality rates among children ⩽5years old was observed, especially in the Northern and Northeastern Brazil. In the current study the 11 gene segments of six Brazilian G1P[6] RVA strains, isolated in 2009 and 2010 from vaccinated children, were analyzed in order to investigate if the genetic composition of these strains might help to elucidate why they were able to cause acute gastroenteritis in vaccinated children. All six Brazilian RVA strains revealed a complete Wa-like genotype constellation: G1-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Phylogenetic analysis showed that all six strains were nearly identical and showed a close genetic relationship with contemporary typical human Wa-like RVA strains. These results suggests that the fact that these strains were able to cause acute gastroenteritis in vaccinated children is likely not due to the genetic background of the strains, but rather to other factors such as host relating factors, co-infecting pathogens or vaccine efficacy. P[6] RVA strains are detected rather occasionally in humans in most regions of the world, except for South Asia and Sub-Saharan Africa. However, recently two studies conducted in Brazil showed the circulation of G12P[6] and G2P[6]. This is the first report on the detection and complete genome analyses of G1P[6] RVA strains in Brazil. Surveillance studies will be crucial to further investigate the prevalence of this genotype in the Brazilian population, and the efficacy of current licensed vaccines, which do not contain the P[6] genotype. |
BibTeX: @article{Gomez2013, author = {Gómez, Mariela Martínez and da Silva, Marcelle Figueira Marques and Zeller, Mark and Heylen, Elisabeth and Matthijnssens, Jelle and Ichihara, Maria Yuri Travassos and Rose, Tatiana Lundgren and de Mello Volotão, Eduardo and Leite, Jose Paulo Gagliardi}, title = {Phylogenetic analysis of G1P[6] group A rotavirus strains detected in Northeast Brazilian children fully vaccinated with Rotarix™.}, journal = {Infect Genet Evol}, year = {2013}, volume = {19}, pages = {395--402}, url = {http://dx.doi.org/10.1016/j.meegid.2013.03.028}, doi = {10.1016/j.meegid.2013.03.028} } |
| Leon LAA (), "Saliva specimen sampling: a noninvasive method for diagnosis and basic investigation of viral hepatitis A, B and C", Future Virology. [DOI] |
| Abstract: Saliva is a biological fluid that is easy to collect and manipulate. Collection of saliva samples is less expensive, noninvasive and painless compared with blood collection. Due to these advantages, saliva has been investigated as an alternative fluid to serum for diagnostic and epidemiological purposes. The aim of this article was to the review research on salivary biomarkers of viral hepatitis A, B and C, highlighting their current use, collection devices, and potential applications for diagnosis and epidemiological studies. This paper also explores recent findings of saliva as a possible source of viral hepatitis transmission. |
BibTeX: @article{Leon, author = {Leon, L. A. A.}, title = {Saliva specimen sampling: a noninvasive method for diagnosis and basic investigation of viral hepatitis A, B and C}, journal = {Future Virology}, doi = {10.2217/fvl.13.41} } |
| Pires MS, dos Santos TM, Santos HA, Vilela JAR, Peixoto MP, Roier ECR, da Silva CB, Barreira JD, de Lemos ERS and Massard CL (2013), "Amblyomma cajennense infestation on horses in two microregions of the state of Rio de Janeiro, Brazil", Revista Brasileira De Parasitologia Veterinaria., April, 2013. Vol. 22(2), pp. 235-242. Brazilian Coll Veterinary Parasitology. [DOI] |
| Abstract: The objective of this study was to evaluate factors associated with infestation by Amblyomma cajennense on horses in two microregions of the state of Rio de Janeiro. Horses on 62 farms in the municipalities of the Itaguai and Serrana microregions were evaluated between January and May 2009. The animals were examined to determine the presence of ticks and infestation level. The animals' rearing and management were assessed on each farm property using an epidemiological questionnaire. Out of the 635 horses evaluated, 41.6% were infested with A. cajennense. It was observed that farms in low-altitude regions (OR=3.69; CI: 2.3-5.8), with unsatisfactory zootechnical and sanitary management (OR=5.92; CI: 3.8-9.2) and an extensive rearing system (OR=4.25; CI: 2.1-8.5) were factors associated with tick infestation (p < 0.05) and also with cases of high infestation on horses. Use of chemical acaricides on horses was also associated with infestation (p < 0.05); the owners described different therapeutic approaches with different treatment intervals. From the present study, low altitudes, unsatisfactory management, extensive rearing and inappropriate use of acaricide products were factors associated with occurrences of A. cajennense at different infestation levels on horses in these municipalities. |
BibTeX: @article{Pires2013, author = {Pires, M. S. and dos Santos, T. M. and Santos, H. A. and Vilela, J. A. R. and Peixoto, M. P. and Roier, E. C. R. and da Silva, C. B. and Barreira, J. D. and de Lemos, E. R. S. and Massard, C. L.}, title = {Amblyomma cajennense infestation on horses in two microregions of the state of Rio de Janeiro, Brazil}, journal = {Revista Brasileira De Parasitologia Veterinaria}, publisher = {Brazilian Coll Veterinary Parasitology}, year = {2013}, volume = {22}, number = {2}, pages = {235--242}, doi = {10.1590/S1984-29612013005000017} } |
| Prado T, Guilayn WDPB, Gaspar AMC and Miagostovich MP (2013), "The efficiency of concentration methods used to detect enteric viruses in anaerobically digested sludge", Memorias Do Instituto Oswaldo Cruz., February, 2013. Vol. 108(1), pp. 77-83. Fundaco Oswaldo Cruz. [DOI] |
| Abstract: The presence of enteric viruses in biosolids can be underestimated due to the inefficient methods (mainly molecular methods) used to recover the viruses from these matrices. Therefore, the goal of this study was to evaluate the different methods used to recover adenoviruses (AdV), rotavirus species A (RVA), norovirus genogroup II (NoV GII) and the hepatitis A virus (HAV) from biosolid samples at a large urban wastewater treatment plant in Brazil after they had been treated by mesophilic anaerobic digestion. Quantitative polymerase chain reaction (PCR) was used for spiking experiments to compare the detection limits of feasible methods, such as beef extract elution and ultracentrifugation. Tests were performed to detect the inhibition levels and the bacteriophage PP7 was used as an internal control. The results showed that the inhibitors affected the efficiency of the PCR reaction and that beef extract elution is a suitable method for detecting enteric viruses, mainly AdV from biosolid samples. All of the viral groups were detected in the biosolid samples: AdV (90%), RVA, NoV GII (45%) and HAV (18%), indicating the viruses' resistance to the anaerobic treatment process. This is the first study in Brazil to detect the presence of RVA, AdV, NoV GII and HAV in anaerobically digested sludge, highlighting the importance of adequate waste management. |
BibTeX: @article{Prado2013, author = {Prado, T. and Guilayn, W. D. P. B. and Gaspar, A. M. C. and Miagostovich, M. P.}, title = {The efficiency of concentration methods used to detect enteric viruses in anaerobically digested sludge}, journal = {Memorias Do Instituto Oswaldo Cruz}, publisher = {Fundaco Oswaldo Cruz}, year = {2013}, volume = {108}, number = {1}, pages = {77--83}, doi = {10.1590/S0074-02762013000100013} } |
| Santos Cruz M, Andrade T, Bastos FI, Leal E, Bertoni N, Villar LM, Tiesmaki M and Fischer B (2013), "Key drug use, health and socio-economic characteristics of young crack users in two Brazilian cities.", Int J Drug Policy., Sep, 2013. Vol. 24(5), pp. 432-438. [DOI] |
| Abstract: Crack use constitutes a major problem in cities across Brazil. While existing data suggest that crack use is generally concentrated among disenfranchised young people with extensive health problems and crime involvement, extensive data gaps exist. To address this issue, this study aimed to assess key characteristics of young crack users in two Brazilian cities.N=160 regular and young adult (ages 18-24) crack users were recruited by community-based methods in the cities of Rio de Janeiro (Southeast) and Salvador (Northeast). Assessments included an interviewer-administered questionnaire on key social, drug use, health and service use characteristics, as well as serological testing of HBV, HCV and HIV status, and were conducted anonymously between November 2010 and June 2011. Participants provided informed consent and received transportation vouchers following assessment completion. The study was approved by institutional ethics review boards.The majority of participants were: male, with less than high school education, unstably housed (Rio only); gained income from legal or illegal work; arrested by police in past year (Salvador only); had numerous daily crack use episodes and shared paraphernalia (Salvador only); co-used alcohol, tobacco, cannabis and cocaine; had no injection history; rated physical and mental health as 'fair' or lower (Salvador only); had unprotected sex; were never HIV tested; were not HIV, HBV or HCV positive; and did not use existing social or health services, but desired access to crack user specific services.Crack users in the two Brazilian sites featured extensive socio-economic marginalization, crack and poly-drug use as well as sexual risk behaviours, and compromised health status. Social and health service utilization are low, yet needs are high. There is an urgent need for further research and for targeted interventions for crack use in Brazil. |
BibTeX: @article{SantosCruz2013, author = {Santos Cruz, Marcelo and Andrade, Tarcisio and Bastos, Francisco I. and Leal, Erotildes and Bertoni, Neilane and Villar, Livia Melo and Tiesmaki, Maija and Fischer, Benedikt}, title = {Key drug use, health and socio-economic characteristics of young crack users in two Brazilian cities.}, journal = {Int J Drug Policy}, year = {2013}, volume = {24}, number = {5}, pages = {432--438}, url = {http://dx.doi.org/10.1016/j.drugpo.2013.03.012}, doi = {10.1016/j.drugpo.2013.03.012} } |
| Vicentini F, Denadai W, Gomes YM, Rose TL, Ferreira MSR, Le Moullac-Vaidye B, Le Pendu J, Leite JPG, Miagostovich MP and Spano LC (), "Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil", Plos One. [DOI] |
| Abstract: Noroviruses (NoV) are the main etiological agents of gastroenteritis outbreaks worldwide and susceptibility to NoV infection has been related to the histo-blood group antigen (HBGA). This study aimed to determine the prevalence of NoV strains and to evaluate the HBGA phenotype and genotype of children from semi-isolated Quilombola communities, descendents of black slaves in Brazil. A total of 397 children up to eleven years old, with and without diarrhea, from Quilombola Communities in the Espirito Santo State, Brazil, were investigated for the presence of NoV from August 2007 to September 2009. Feces were collected from all the children, and blood from the NoV positive children. NoV was screened by reverse transcription-PCR with primers for the RNA-dependent RNA polymerase region; genogroup was determined by PCR with primers for the C and D regions and genotyped by sequencing. HBGA phenotype was performed by gel-spinning and FUT2 and FUT3 were analyzed by PCR or sequencing analysis. NoV were detected in 9.2% (12/131) of diarrheic and 1.5% (4/266) of non-diarrheic children (p<0.05, Fishers exact test). GI and GII genogroups were present in 12.5% and 87.5% of the samples, respectively. The following genotypes were characterized: GII.4 (25%), GII.12 (25%), GII.6 (12.5%) and GI.1 (6.3%), GI.3 (12.5%) and GI.4 (6.3%). Children infected with NoV showed the A (n = 6), O (n = 6), and B (n = 2) HBGA phenotypes, and 13 of them were classified as secretors (Se) and one as a non secretor (se). Mutations of Se-40,Se- 171,Se-216,Se-357,Se-428,Se-739,Se-960 were found for the FUT2 gene and mutations of Le(59, 202, 314) for the FUT3 gene. The only se child was infected by NoV GI, whereas the Se children were indiscriminately infected by GI or GII. This study showed rates of NoV infection in symptomatic and asymptomatic Quilombola children consistent with other studies. However, children under 12 months were seven times more affected than those between 1 and 5 years old. GII.12 was as frequent as GII.4 and GI.1 and GI.4 were described for the first time in Brazil. Owing to the small number of cases studied, no clear pattern of susceptibility and/or HBGA resistance could be inferred. |
BibTeX: @article{Vicentini, author = {Vicentini, F. and Denadai, W. and Gomes, Y. M. and Rose, T. L. and Ferreira, M. S. R. and Le Moullac-Vaidye, B. and Le Pendu, J. and Leite, J. P. G. and Miagostovich, M. P. and Spano, L. C.}, title = {Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil}, journal = {Plos One}, doi = {10.1371/journal.pone.0069348} } |
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