Área de PDI em Pesquisa, Desenvolvimento e Inovação em Doenças Bacterianas e Fúngicas 2013
Veja, abaixo, a relação de artigos científicos publicados pelo IOC, na referida Área Temática, organizados em ordem alfabética crescente:
| Amaral JJ, Antunes LCM, de Macedo CS, Mattos KA, Han J, Pan JX, Candea ALP, Henriques MDMO, Ribeiro-Alves M, Borchers CH, Sarno EN, Bozza PT, Finlay BB and Pessolani MCV (2013), "Metabonomics Reveals Drastic Changes in Anti-Inflammatory/Pro-Resolving Polyunsaturated Fatty Acids-Derived Lipid Mediators in Leprosy Disease", Plos Neglected Tropical Diseases., August, 2013. Vol. 7(8), pp. e2381. Public Library Science. [DOI] |
| Abstract: Despite considerable efforts over the last decades, our understanding of leprosy pathogenesis remains limited. The complex interplay between pathogens and hosts has profound effects on host metabolism. To explore the metabolic perturbations associated with leprosy, we analyzed the serum metabolome of leprosy patients. Samples collected from lepromatous and tuberculoid patients before and immediately after the conclusion of multidrug therapy (MDT) were subjected to high-throughput metabolic profiling. Our results show marked metabolic alterations during leprosy that subside at the conclusion of MDT. Pathways showing the highest modulation were related to polyunsaturated fatty acid (PUFA) metabolism, with emphasis on anti-inflammatory, pro-resolving omega-3 fatty acids. These results were confirmed by eicosanoid measurements through enzyme-linked immunoassays. Corroborating the repertoire of metabolites altered in sera, metabonomic analysis of skin specimens revealed alterations in the levels of lipids derived from lipase activity, including PUFAs, suggesting a high lipid turnover in highly-infected lesions. Our data suggest that omega-6 and omega-3, PUFA-derived, pro-resolving lipid mediators contribute to reduced tissue damage irrespectively of pathogen burden during leprosy disease. Our results demonstrate the utility of a comprehensive metabonomic approach for identifying potential contributors to disease pathology that may facilitate the development of more targeted treatments for leprosy and other inflammatory diseases. |
BibTeX:@article{Amaral2013, author = {Amaral, J. J. and Antunes, L. C. M. and de Macedo, C. S. and Mattos, K. A. and Han, J. and Pan, J. X. and Candea, A. L. P. and Henriques, M. D. M. O. and Ribeiro-Alves, M. and Borchers, C. H. and Sarno, E. N. and Bozza, P. T. and Finlay, B. B. and Pessolani, M. C. V.}, title = {Metabonomics Reveals Drastic Changes in Anti-Inflammatory/Pro-Resolving Polyunsaturated Fatty Acids-Derived Lipid Mediators in Leprosy Disease}, journal = {Plos Neglected Tropical Diseases}, publisher = {Public Library Science}, year = {2013}, volume = {7}, number = {8}, pages = {e2381}, doi = {10.1371/journal.pntd.0002381}} |
| Arnaldo P, Thompson RE, Lopes MQ, Suffys PN and Santos AR (2013), "Frequencies of Cytochrome P450 2B6 and 2C8 Allelic Variants in the Mozambican Population.", Malays J Med Sci., Jul, 2013. Vol. 20(4), pp. 13-23. |
| Abstract: The cytochrome P450 enzymes (CYP) play an important role in the metabolism of many therapeutic agents. The activities of different enzymes exhibit variability in different populations, which causes variations in drug response or toxicity. The CYP2B6 and CYP2C8 enzymes are encoded by polymorphic genes characterised by different single nucleotide polymorphisms (SNPs). Several of these CYP variants are often associated with slow metabolism phenotypes. This study aimed to analyse the frequencies of allelic variants of CYP2B6 and CYP2C8 in the Mozambican population.Using a polymerase chain reaction and restriction fragment length polymorphism assay (PCR-RFLP), the frequencies of the allelic variants of CYP2B6 (c.64C>T, c.516G>T, c.777C>A, c.785A>G, c.1459C>T) and CYP2C8 (c.805A>T, c.416G>A, c.1196A>G, c.792C>G) were determined in 360 Mozambican blood donors.The frequencies of the allelic variants of the CYP2B6 gene were 0.057, 0.426, 0.0, 0.410, and 0.004. For the CYP2C8 gene, the frequencies of the allelic variants were 0.160, 0.048, 0.0, and 0.005. No significant differences were observed between the gender and geographic distribution of volunteers around the country.The frequencies of the allelic variants of the CYP2B6 and CYP2C8 genes were found to be homogeneously distributed in the Mozambican population and were comparable to other African populations. Further studies are required to explore the impact of these variants on the clinical response (efficacy and toxicity) of drugs, including antimalarials. |
BibTeX:@article{Arnaldo2013, author = {Arnaldo, Paulo and Thompson, Ricardo Estevão and Lopes, Márcia Quinhones and Suffys, Philip Noel and Santos, Adalberto Rezende}, title = {Frequencies of Cytochrome P450 2B6 and 2C8 Allelic Variants in the Mozambican Population.}, journal = {Malays J Med Sci}, year = {2013}, volume = {20}, number = {4}, pages = {13--23}} |
| Barroso DE, Castineiras TMPP, Cabral AC, Vicente ACP, Rebelo MC, Cerqueira EO, Tulenko MM, Marsh JW, Krauland MG and Harrison LH (2013), "Neisseria meningitidis ST-11 clonal complex bearing capsule serogroups B, C, or W in Brazil", Journal of Infection., June, 2013. Vol. 66(6), pp. 547-550. W B Saunders Co Ltd. [DOI] |
BibTeX:@article{Barroso2013, author = {Barroso, D. E. and Castineiras, T. M. P. P. and Cabral, A. C. and Vicente, A. C. P. and Rebelo, M. C. and Cerqueira, E. O. and Tulenko, M. M. and Marsh, J. W. and Krauland, M. G. and Harrison, L. H.}, title = {Neisseria meningitidis ST-11 clonal complex bearing capsule serogroups B, C, or W in Brazil}, journal = {Journal of Infection}, publisher = {W B Saunders Co Ltd}, year = {2013}, volume = {66}, number = {6}, pages = {547--550}, doi = {10.1016/j.jinf.2013.01.002}} |
| Bastos ML, Menzies D, Belo MTCT, Teixeira EG, de Abreu ST, Antas PRZ and Trajman A (2013), "Changes in QuantiFERON (R)-TB Gold In-Tube results during treatment for tuberculous infection", International Journal of Tuberculosis and Lung Disease., July, 2013. Vol. 17(7), pp. 909-916. Int Union Against Tuberculosis Lung Disease (i U A T L D). [DOI] |
| Abstract: SETTING: Randomised trial comparing 9 months of isoniazid with 4 months of rifampicin for the treatment of high-risk tuberculin skin test positive subjects in Rio de Janeiro, Brazil. OBJECTIVES: To compare QuantiFERON (R)-TB Gold In-Tube (QFT-GIT) responses before and 1,4 and 9 months after starting treatment for latent tuberculous infection (LTBI) according to adherence to one of the two regimens. DESIGN: Participants in the trial were invited to undergo serial QFT-GIT. Within-subject differences at different time points were analysed as quantitative responses and categorised as positive or negative using different cut-off points. RESULTS: Of 215 participants, 118 completed treatment, of whom 58 underwent all three tests; and 97 did not complete treatment, of whom 10 underwent all tests. After 1 month of treatment, there was no significant difference in QFT-GIT response between the groups. After 4 and 9 months, reversions were more frequent in non-adherent subjects. Marked within-subject fluctuations were observed. No cut-off point could be established at which QFT-GIT responses were consistently positive or associated with adherence or type of treatment. CONCLUSION: Frequent within-subject variability in QFT-GIT responses, not associated with LTBI treatment, makes it difficult for clinicians to interpret QFT-GIT conversions and reversions. |
BibTeX:@article{Bastos2013, author = {Bastos, M. L. and Menzies, D. and Belo, M. T. C. T. and Teixeira, E. G. and de Abreu, S. T. and Antas, P. R. Z. and Trajman, A.}, title = {Changes in QuantiFERON (R)-TB Gold In-Tube results during treatment for tuberculous infection}, journal = {International Journal of Tuberculosis and Lung Disease}, publisher = {Int Union Against Tuberculosis Lung Disease (i U A T L D)}, year = {2013}, volume = {17}, number = {7}, pages = {909--916}, doi = {10.5588/ijtld.12.0927}} |
| Carletti D, da Fonseca DM, Gembre AF, Masson AP, Campos LW, Leite LCC, Pires AR, Lannes-Vieira J, Silva CL, Bonato VLD and Horn C (2013), "A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6 '-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice", Clinical and Vaccine Immunology., August, 2013. Vol. 20(8), pp. 1162-1169. Amer Soc Microbiology. [DOI] |
| Abstract: Mycobacterium bovis BCG prime DNA (Mycobacterium tuberculosis genes)-booster vaccinations have been shown to induce greater protection against tuberculosis (TB) than BCG alone. This heterologous prime-boost strategy is perhaps the most realistic vaccination for the future of TB infection control, especially in countries where TB is endemic. Moreover, a prime-boost regimen using biodegradable microspheres seems to be a promising immunization to stimulate a long-lasting immune response. The alanine proline antigen (Apa) is a highly immunogenic glycoprotein secreted by M. tuberculosis. This study investigated the immune protection of Apa DNA vaccine against intratracheal M. tuberculosis challenge in mice on the basis of a heterologous prime-boost regimen. BALB/c mice were subcutaneously primed with BCG and intramuscularly boosted with a single dose of plasmid carrying apa and 6,6'-trehalose dimycolate (TDM) adjuvant, coencapsulated in microspheres (BCG-APA), and were evaluated 30 and 70 days after challenge. This prime-boost strategy (BCG-APA) resulted in a significant reduction in the bacterial load in the lungs, thus leading to better preservation of the lung parenchyma, 70 days postinfection compared to BCG vaccinated mice. The profound effect of this heterologous prime-boost regimen in the experimental model supports its development as a feasible strategy for prevention of TB. |
BibTeX:@article{Carletti2013, author = {Carletti, D. and da Fonseca, D. M. and Gembre, A. F. and Masson, A. P. and Campos, L. W. and Leite, L. C. C. and Pires, A. R. and Lannes-Vieira, J. and Silva, C. L. and Bonato, V. L. D. and Horn, C.}, title = {A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6 '-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice}, journal = {Clinical and Vaccine Immunology}, publisher = {Amer Soc Microbiology}, year = {2013}, volume = {20}, number = {8}, pages = {1162--1169}, doi = {10.1128/CVI.00148-13}} |
| Chung AW, Sieling PA, Schenk M, Teles RMB, Krutzik SR, Hsu DK, Liu FT, Sarno EN, Rea TH, Stenger S, Modlin RL and Lee DJ (2013), "Galectin-3 Regulates the Innate Immune Response of Human Monocytes", Journal of Infectious Diseases., March, 2013. Vol. 207(6), pp. 947-956. Oxford Univ Press Inc. [DOI] |
| Abstract: Galectin-3 is a beta-galactoside-binding lectin widely expressed on epithelial and hematopoietic cells, and its expression is frequently associated with a poor prognosis in cancer. Because it has not been well-studied in human infectious disease, we examined galectin-3 expression in mycobacterial infection by studying leprosy, an intracellular infection caused by Mycobacterium leprae. Galectin-3 was highly expressed on macrophages in lesions of patients with the clinically progressive lepromatous form of leprosy; in contrast, galectin-3 was almost undetectable in self-limited tuberculoid lesions. We investigated the potential function of galectin-3 in cell-mediated immunity using peripheral blood monocytes. Galectin-3 enhanced monocyte interleukin 10 production to a TLR2/1 ligand, whereas interleukin 12p40 secretion was unaffected. Furthermore, galectin-3 diminished monocyte to dendritic cell differentiation and T-cell antigen presentation. These data demonstrate an association of galectin-3 with unfavorable host response in leprosy and a potential mechanism for impaired host defense in humans. |
BibTeX:@article{Chung2013, author = {Chung, A. W. and Sieling, P. A. and Schenk, M. and Teles, R. M. B. and Krutzik, S. R. and Hsu, D. K. and Liu, F. T. and Sarno, E. N. and Rea, T. H. and Stenger, S. and Modlin, R. L. and Lee, D. J.}, title = {Galectin-3 Regulates the Innate Immune Response of Human Monocytes}, journal = {Journal of Infectious Diseases}, publisher = {Oxford Univ Press Inc}, year = {2013}, volume = {207}, number = {6}, pages = {947--956}, doi = {10.1093/infdis/jis920}} |
| Costa ERD, Lazzarini LCO, Perizzolo PF, Diaz CA, Spies FS, Costa LL, Ribeiro AW, Barroco C, Schuh SJ, Pereira MADS, Dias CF, Gomes HM, Unis G, Zaha A, da Silva PEA, Suffys PN and Rossetti MLR (2013), "Mycobacterium tuberculosis of the RDRio Genotype Is the Predominant Cause of Tuberculosis and Associated with Multidrug Resistance in Porto Alegre City, South Brazil", Journal of Clinical Microbiology., April, 2013. Vol. 51(4), pp. 1071-1077. Amer Soc Microbiology. [DOI] |
| Abstract: Spoligotyping has shown Mycobacterium tuberculosis strains to be composed of different lineages, and some of them are not just geographically restricted but also affect specific ethnic populations and are associated with outbreaks and drug resistance. We recently described a particular subtype within the Latin American-Mediterranean (LAM) family, called RDRio, widespread in Brazil. Moreover, recent data also indicate that RDRio is present in many countries on all continents and is associated with cavitary disease and multidrug resistance (MDR). To further explore the relationship between RDRio and MDR, we conducted a study in a tuberculosis (TB) reference center responsible for the care of MDR patients in Rio Grande do Sul, the southernmost Brazilian state. From a collection of 237 clinical isolates, RDRio alone was responsible for one-half of all MDR cases, including one large group composed of strains with identical IS6110-restriction fragment length polymorphism (RFLP) and having the LAM5 signature. We additionally had complete data records for 96 patients and could make comparisons between the presence and absence of RDRio. No difference in clinical, radiological or laboratory features was observed, but a significantly greater number of cases with MDR were described in patients infected with an RDRio strain (P = 0.0015). Altogether, RDRio was responsible for 38% of all TB cases. These data support and confirmed previous findings that RDRio is the main agent responsible for TB in Brazil and is associated with drug resistance. Considering that RDRio is a globally distributed genotype, such findings raise concern about the increase in MDR in certain human populations. |
BibTeX:@article{Costa2013, author = {Costa, E. R. D. and Lazzarini, L. C. O. and Perizzolo, P. F. and Diaz, C. A. and Spies, F. S. and Costa, L. L. and Ribeiro, A. W. and Barroco, C. and Schuh, S. J. and Pereira, M. A. D. S. and Dias, C. F. and Gomes, H. M. and Unis, G. and Zaha, A. and da Silva, P. E. A. and Suffys, P. N. and Rossetti, M. L. R.}, title = {Mycobacterium tuberculosis of the RDRio Genotype Is the Predominant Cause of Tuberculosis and Associated with Multidrug Resistance in Porto Alegre City, South Brazil}, journal = {Journal of Clinical Microbiology}, publisher = {Amer Soc Microbiology}, year = {2013}, volume = {51}, number = {4}, pages = {1071--1077}, doi = {10.1128/JCM.01511-12}} |
| De Sequeira DCM, Peixoto MLP, De Luca PM, Oliveira-Ferreira J, Antas PRZ and Borba CM (2013), "Detection of antibody to Purpureocillium lilacinum by immunofluorescent assay and flow cytometry in serum of infected C57BL/6 mice.", J Immunol Methods., Oct, 2013. Vol. 396(1-2), pp. 147-151. [DOI] |
| Abstract: Purpureocillium lilacinum is an emerging pathogenic fungus that can cause different clinical manifestations ranging from cutaneous and sub-cutaneous infections to severe oculomycosis. In this study, using both conventional indirect immunofluorescence and non-conventional flow cytometry approaches, IgG antibodies were readily detected in both C57BL/6 immunocompetent and immunosuppressed mice after i.v. infection with P. lilacinum. The humoral immune response was specific, since virtually no antibodies were detected in the serum of control mice. Flow cytometry assays also showed both quantitative and qualitative differences in total IgG and its isotypes in sera of immunocompetent and immunosupressed infected mice. Although a good starting point, it is clear that the effectiveness of serological assays for P. lilacinum hyalohyphomycosis identification in clinical studies still requires further standardization. Upon further validation in humans, these techniques have the potential to be suitable to detect P. lilacinum infection in patients, thereby avoiding current laborious and time-consuming culture techniques. |
BibTeX:@article{deSequeira2013, author = {De Sequeira, Danielly C M. and Peixoto, Mariana L P. and De Luca, Paula M. and Oliveira-Ferreira, Joseli and Antas, Paulo R Z. and Borba, Cintia M.}, title = {Detection of antibody to Purpureocillium lilacinum by immunofluorescent assay and flow cytometry in serum of infected C57BL/6 mice.}, journal = {J Immunol Methods}, year = {2013}, volume = {396}, number = {1-2}, pages = {147--151}, url = {http://dx.doi.org/10.1016/j.jim.2013.07.002}, doi = {10.1016/j.jim.2013.07.002}} |
| Dos Santos DS, Duppre NC, Sales AM, Nery JAdC, Sarno EN and Hacker MA (2013), "Kinship and Leprosy in the Contacts of Leprosy Patients: Cohort at the Souza Araújo Outpatient Clinic, Rio de Janeiro, RJ, 1987-2010.", J Trop Med. Vol. 2013, pp. 596316. [DOI] |
| Abstract: A broad variety of factors have been associated with leprosy among contacts, including socioeconomic, epidemiological, and genetic characteristics. Data from 7,174 contacts of leprosy patients from a leprosy outpatient clinic in Rio de Janeiro, Brazil, 1987-2010, were analyzed to investigate the effects of kinship, individual, and contextual factors on leprosy. Multivariate analyses were performed using a robust estimation method. In the prevalence analysis, close kinship (sibling OR = 2.75, offspring OR = 2.00, and other relatives OR = 1.70), socioeconomic factors, and the duration of exposure to the bacillus were associated to leprosy. In the incidence analysis, significant risks were found for all categories of kinship (parents RR = 10.93, spouse, boyfriend/girlfriend, and bride/groom RR = 7.53, sibling RR = 7.03, offspring RR = 5.34, and other relatives RR = 3.71). Once the treatment of the index case was initiated, other factors lost their significance, and the index case bacteriological index and BCG (Bacillus Calmette-Guérin vaccine) protection had a greater impact. Our findings suggested that both genetic susceptibility and physical exposure play an important role in the epidemiology of leprosy, but it was not possible establishing the role of genetic factor. Analyses of other factors related to the genotype of individuals, such as genetic polymorphisms, are needed. |
BibTeX:@article{DosSantos2013, author = {Dos Santos, Daiane Santos and Duppre, Nadia Cristina and Sales, Anna Maria and Nery, José Augusto da Costa and Sarno, Euzenir Nunes and Hacker, Mariana Andréa}, title = {Kinship and Leprosy in the Contacts of Leprosy Patients: Cohort at the Souza Araújo Outpatient Clinic, Rio de Janeiro, RJ, 1987-2010.}, journal = {J Trop Med}, year = {2013}, volume = {2013}, pages = {596316}, url = {http://dx.doi.org/10.1155/2013/596316}, doi = {10.1155/2013/596316}} |
| Guerreiro LTA, Robottom-Ferreira AB, Ribeiro-Alves M, Toledo-Pinto TG, Brito TR, Rosa PS, Sandoval FG, Jardim MR, Antunes SG, Shannon EJ, Sarno EN, Pessolani MCV, Williams DL and Moraes MO (2013), "Gene Expression Profiling Specifies Chemokine, Mitochondrial and Lipid Metabolism Signatures in Leprosy", Plos One., June, 2013. Vol. 8(6), pp. e64748. Public Library Science. [DOI] |
| Abstract: Herein, we performed microarray experiments in Schwann cells infected with live M. leprae and identified novel differentially expressed genes (DEG) in M. leprae infected cells. Also, we selected candidate genes associated or implicated with leprosy in genetic studies and biological experiments. Forty-seven genes were selected for validation in two independent types of samples by multiplex qPCR. First, an in vitro model using THP-1 cells was infected with live Mycobacterium leprae and M. bovis bacillus Calmette-Guerin (BCG). In a second situation, mRNA obtained from nerve biopsies from patients with leprosy or other peripheral neuropathies was tested. We detected DEGs that discriminate M. bovis BCG from M. leprae infection. Specific signatures of susceptible responses after M. leprae infection when compared to BCG lead to repression of genes, including CCL2, CCL3, IL8 and SOD2. The same 47-gene set was screened in nerve biopsies, which corroborated the down-regulation of CCL2 and CCL3 in leprosy, but also evidenced the down-regulation of genes involved in mitochondrial metabolism, and the up-regulation of genes involved in lipid metabolism and ubiquitination. Finally, a gene expression signature from DEG was identified in patients confirmed of having leprosy. A classification tree was able to ascertain 80% of the cases as leprosy or non-leprous peripheral neuropathy based on the expression of only LDLR and CCL4. A general immune and mitochondrial hypo-responsive state occurs in response to M. leprae infection. Also, the most important genes and pathways have been highlighted providing new tools for early diagnosis and treatment of leprosy. |
BibTeX:@article{Guerreiro2013, author = {Guerreiro, L. T. A. and Robottom-Ferreira, A. B. and Ribeiro-Alves, M. and Toledo-Pinto, T. G. and Brito, T. R. and Rosa, P. S. and Sandoval, F. G. and Jardim, M. R. and Antunes, S. G. and Shannon, E. J. and Sarno, E. N. and Pessolani, M. C. V. and Williams, D. L. and Moraes, M. O.}, title = {Gene Expression Profiling Specifies Chemokine, Mitochondrial and Lipid Metabolism Signatures in Leprosy}, journal = {Plos One}, publisher = {Public Library Science}, year = {2013}, volume = {8}, number = {6}, pages = {e64748}, doi = {10.1371/journal.pone.0064748}} |
| Jaeger LH, de Souza SMFM, Dias OF and Iñiguez AM (2013), "Mycobacterium tuberculosis complex in remains of 18th-19th century slaves, Brazil.", Emerg Infect Dis., May, 2013. Vol. 19(5), pp. 837-839. [DOI] |
BibTeX:@article{Jaeger2013, author = {Jaeger, Lauren H. and de Souza, Sheila M F M. and Dias, Ondemar F. and Iñiguez, Alena M.}, title = {Mycobacterium tuberculosis complex in remains of 18th-19th century slaves, Brazil.}, journal = {Emerg Infect Dis}, year = {2013}, volume = {19}, number = {5}, pages = {837--839}, url = {http://dx.doi.org/10.3201/eid1905.120193}, doi = {10.3201/eid1905.120193}} |
| Luiz RDS, Suffys P, Barroso EC, Kerr LRFS, Duarte CR, Freitas MVC, Mota RMS and Frota CC (2013), "Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil", Brazilian Journal of Infectious Diseases., May, 2013. Vol. 17(3), pp. 338-345. Contexto. [DOI] |
| Abstract: Objectives: This study has used a combination of clinical information, spoligotyping, and georeferencing system to elucidate the genetic diversity of the Mycobacterium tuberculosis isolates circulating in a TB-prevalent municipality of Northeast Brazil. Methods: A total of 115 M. tuberculosis strains were isolated from pulmonary tuberculosis patients from January 2007 to March 2008 in Fortaleza. Drug susceptibility and spoligotyping assays were performed and place of residence of the patients were georeferenced. Results: Of the M. tuberculosis strains studied, 51 (44.3%) isolates were resistant to at least one drug (R-TB) and 64 (55.7%) were sensitive to all the drugs tested (S-TB). A high frequency of resistancewas found in previously treated cases (84%) and among newcases (16%; p < 0.001). A total of 74 (64%) isolates were grouped into 22 spoligotyped lineages, while 41 (36%) isolates were identified as new. Among the predominant genotypes, 33% were Latim American Mediterranean (LAM), 12% Haarlem (H), and 5% U. There was no association of geographic distribution of RT-TB patients as compared to the controls and also the geographic location to the spoligotype patterns. The geospatial analysis revealed that 24 (23%) patients (hot spot zones) either shared the same residence or lived in a close neighborhood of a case. Among these concentration zones, the patients lived in the same residence and shared a common genotype pattern and resistance pattern. Discussion: It was observed that the spoligopatterns family distribution was similar to that reported for South America, prevailing the LAM and H lineages. A high rate-case among the resistant TB group occurs as a result of transmitted and acquired resistance. Amore effective surveillance program is needed in order to succeed in reducing tuberculosis in Northeast Brazil. (c) 2013 Elsevier Editora Ltda. All rights reserved. |
BibTeX:@article{Luiz2013, author = {Luiz, R. D. S. and Suffys, P. and Barroso, E. C. and Kerr, L. R. F. S. and Duarte, C. R. and Freitas, M. V. C. and Mota, R. M. S. and Frota, C. C.}, title = {Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil}, journal = {Brazilian Journal of Infectious Diseases}, publisher = {Contexto}, year = {2013}, volume = {17}, number = {3}, pages = {338--345}, doi = {10.1016/j.bjid.2012.10.019}} |
| Marin MA, Thompson CC, Freitas FS, Fonseca EL, Aboderin AO, Zailani SB, Quartey NKE, Okeke IN and Vicente ACP (2013), "Cholera Outbreaks in Nigeria Are Associated with Multidrug Resistant Atypical El Tor and Non-O1/Non-O139 Vibrio cholerae", Plos Neglected Tropical Diseases., February, 2013. Vol. 7(2), pp. e2049. Public Library Science. [DOI] |
| Abstract: Background: The current millennium has seen a steep rise in the number, size and case-fatalities of cholera outbreaks in many African countries. Over 40,000 cases of cholera were reported from Nigeria in 2010. Variants of Vibrio cholerae O1 El Tor biotype have emerged but very little is known about strains causing cholera outbreaks in West Africa, which is crucial for the implementation of interventions to control epidemic cholera. Methodology/Principal Findings: V. cholerae isolates from outbreaks of acute watery diarrhea in Nigeria from December, 2009 to October, 2010 were identified by standard culture methods. Fifteen O1 and five non-O1/non-O139 strains were analyzed; PCR and sequencing targeted regions associated with virulence, resistance and biotype were performed. We also studied genetic interrelatedness among the strains by multilocus sequence analysis and pulsed-field gel electrophoresis. The antibiotic susceptibility was tested by the disk diffusion method and E-test. We found that multidrug resistant atypical El Tor strains, with reduced susceptibility to ciprofloxacin and chloramphenicol, characterized by the presence of the SXT element, and gyrA Ser83Ile /parC Ser85Leu alleles as well CTX phage and TCP cluster characterized by rstR ElTor, ctxB-7 and tcpA CIRS alleles, respectively, were largely responsible for cholera outbreaks in 2009 and 2010. We also identified and characterized a V. cholerae non-O1/non-O139 lineage from cholera-like diarrhea cases in Nigeria. Conclusions/Significance: The recent Nigeria outbreaks have been determined by multidrug resistant atypical El Tor and non-O1/non-O139 V. cholerae strains, and it seems that the typical El Tor, from the beginning of seventh cholera pandemic, is no longer epidemic/endemic in this country. This scenario is similar to the East Africa, Asia and Caribbean countries. The detection of a highly virulent, antimicrobial resistant lineage in Nigeria is worrisome and points to a need for vaccine-based control of the disease. This study has also revealed the putative importance of non-O1/non-O139 V. cholerae in diarrheal disease in Nigeria. |
BibTeX:@article{Marin2013, author = {Marin, M. A. and Thompson, C. C. and Freitas, F. S. and Fonseca, E. L. and Aboderin, A. O. and Zailani, S. B. and Quartey, N. K. E. and Okeke, I. N. and Vicente, A. C. P.}, title = {Cholera Outbreaks in Nigeria Are Associated with Multidrug Resistant Atypical El Tor and Non-O1/Non-O139 Vibrio cholerae}, journal = {Plos Neglected Tropical Diseases}, publisher = {Public Library Science}, year = {2013}, volume = {7}, number = {2}, pages = {e2049}, doi = {10.1371/journal.pntd.0002049}} |
| Mendes-Marques CL, Hofer E and Leal NC (2013), "Development of duplex-PCR for identification of Aeromonas species", Revista Da Sociedade Brasileira De Medicina Tropical., May, 2013. Vol. 46(3), pp. 355-357. Soc Brasileira Medicina Tropical. [DOI] |
| Abstract: Introduction: The number of reports of intestinal infections caused by Aeromonas spp. has increased significantly in recent years. In most clinical laboratories, identification of these bacteria is carried out by general phenotypic tests that sometimes do not accurately differentiate Aeromonas and Vibrio. Methods: A duplex-polymerase chain reaction (PCR) was developed directed to 2 targets identifying Aeromonas spp. pathogenic to humans. Results: The duplex-PCR results were reproducible and specific for Aeromonas spp. pathogenic to humans. Conclusions: This method will allow differentiation between Vibrio and Aeromonas spp. in patients with in cholera-like symptoms and can also be used in water quality monitoring. |
BibTeX:@article{Mendes-Marques2013a, author = {Mendes-Marques, C. L. and Hofer, E. and Leal, N. C.}, title = {Development of duplex-PCR for identification of Aeromonas species}, journal = {Revista Da Sociedade Brasileira De Medicina Tropical}, publisher = {Soc Brasileira Medicina Tropical}, year = {2013}, volume = {46}, number = {3}, pages = {355--357}, doi = {10.1590/0037-8682-1344-2013}} |
| Mendes-Marques CL, Silveira VD, da Costa APR, Nunes MD, da Silva SV, Figueiroa ACTD, Hofer E, de Almeida AMP and Leal NC (2013), "The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil", Scientific World Journal. , pp. 746254. Hindawi Publishing Corporation. [DOI] |
| Abstract: After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were also ctxA (cholera toxin) positive. From May to June 2012, 30 V. cholerae O1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for the rfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic V. cholerae O1 in the region. |
BibTeX:@article{Mendes-Marques2013, author = {Mendes-Marques, C. L. and Silveira, V. D. and da Costa, A. P. R. and Nunes, M. D. and da Silva, S. V. and Figueiroa, A. C. T. D. and Hofer, E. and de Almeida, A. M. P. and Leal, N. C.}, title = {The Aquatic Environment as a Reservoir of Vibrio cholerae O1 in Hydrographic Basins of the State of Pernambuco, Brazil}, journal = {Scientific World Journal}, publisher = {Hindawi Publishing Corporation}, year = {2013}, pages = {746254}, doi = {10.1155/2013/746254}} |
| Paixao R, Moreno LZ, de Gobbi DDS, Raimundo DC, Hofer E, Matte MH, Ferreira TSP, Gomes VTD, Costa BLP and Moreno AM (2013), "Characterization of Yersinia enterocolitica Biotype 1A Strains Isolated from Swine Slaughterhouses and Markets", Scientific World Journal. , pp. 769097. Hindawi Publishing Corporation. [DOI] |
| Abstract: Yersinia enterocolitica is an important foodborne pathogen that causes illness in humans and animals. Y. enterocolitica is also the most heterogeneous species of the genus and is divided into distinct serotypes and over six biotypes. Y. enterocolitica biotype 1A strains are classically considered as nonpathogenic; however, some biotype 1A isolates have been considered as causative of gastrointestinal disease, yielding symptoms indistinguishable from those produced by pathogenic biotypes. Even after decades of isolation of clinical strains, the pathogenic mechanisms of these isolates are still not fully understood. In the present study, 122 Yersinia enterocolitica biotype 1A strains isolated from swine slaughterhouses and meat markets in Sao Paulo, Brazil, were characterized according to the presence of the virulence genes ail, virF, and ystA. A total of 94 strains were positive to at least one virulence gene (77.05%), and 67 were positive to all of them (54.92%). Twenty-two strains were submitted to PFGE genotyping resulting in 22 distinct pulsotypes, varying from 50% to 84% of genetic similarity. Any clustering tendency among pulsotypes related to origin, isolation site, or even virulence profile was not observed. The present study reports an important contamination of the environment in swine slaughterhouses, meat markets, and pork, by potentially virulent Y. enterocolitica biotype 1A. |
BibTeX:@article{Paixao2013, author = {Paixao, R. and Moreno, L. Z. and de Gobbi, D. D. S. and Raimundo, D. C. and Hofer, E. and Matte, M. H. and Ferreira, T. S. P. and Gomes, V. T. D. and Costa, B. L. P. and Moreno, A. M.}, title = {Characterization of Yersinia enterocolitica Biotype 1A Strains Isolated from Swine Slaughterhouses and Markets}, journal = {Scientific World Journal}, publisher = {Hindawi Publishing Corporation}, year = {2013}, pages = {769097}, doi = {10.1155/2013/769097}} |
| Paixão R, Moreno LZ, Sena de Gobbi DD, Raimundo DC, Ferreira TSP, Spindola MG, Hofer E, Falavina Dos Reis CM, Matté MH and Micke Moreno A (2013), "Genotypic Characterization of Yersinia enterocolitica Biotype 4/O:3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE.", J Pathog. Vol. 2013, pp. 521510. [DOI] |
| Abstract: Yersinia enterocolitica is a foodborne pathogen that causes illness in humans and animals. The biotype 4/O:3 has been commonly associated with yersiniosis and is characterized by the presence of chromosomal and extra-chromosomal virulence genes. Molecular typing methods have been successfully used to characterize Y. enterocolitica genetic heterogeneity and to study the epidemiology of the bacteria from different origins. In this study, 320 Y. enterocolitica biotype 4/O:3 isolates originating in pigs and slaughterhouses were characterized according to the virulence profile, and 61 isolates were typified through SE-AFLP, ERIC-PCR, and PFGE techniques. The majority of the isolates originated from pigs, and the predominant virulence profile was ail+ virF+ rfbC+ ystA+, representing 83.4% of the tested isolates. All of the Y. enterocolitica 4/O:3 isolates were positive for at least ystA gene. The SE-AFLP and ERIC-PCR patterns were highly homogeneous. The SE-AFLP was more discriminative than the ERIC-PCR and tended to cluster isolates according to the slaughterhouse. Despite the limited genetic diversity of Y. enterocolitica 4/O:3, PFGE was shown to be the most discriminative technique considering one band of difference. Fattening pigs proved to be an important reservoir of Y. enterocolitica biotype 4/O:3 carrying virulence genes. |
BibTeX:@article{Paixao2013a, author = {Paixão, Renata and Moreno, Luisa Zanolli and Sena de Gobbi, Débora Dirani and Raimundo, Daniele Cristine and Ferreira, Thais Sebastiana Porfida and Spindola, Maria Garcia and Hofer, Ernesto and Falavina Dos Reis, Cristhiane Moura and Matté, Maria Helena and Micke Moreno, Andrea}, title = {Genotypic Characterization of Yersinia enterocolitica Biotype 4/O:3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE.}, journal = {J Pathog}, year = {2013}, volume = {2013}, pages = {521510}, url = {http://dx.doi.org/10.1155/2013/521510}, doi = {10.1155/2013/521510}} |
| Pereira PS, de Araujo CFM, Seki LM, Zahner V, Carvalho-Assef APD and Asensi MD (2013), "Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340)", Journal of Antimicrobial Chemotherapy., February, 2013. Vol. 68(2), pp. 312-316. Oxford Univ Press. [DOI] |
| Abstract: To perform molecular epidemiology for 113 KPC-producing Klebsiella pneumoniae isolated in 2010 from 12 Brazilian states. The resistance profile was determined by disc diffusion and Etest. Genetic polymorphism was analysed by PFGE and multilocus sequence typing. The genetic environment of the bla(KPC) gene was determined by PCR and identification of the carrier plasmid was determined by hybridization. Most of the isolates were multidrug resistant, with 15 and 49 being resistant to polymyxin and tigecycline, respectively. Twenty-two sequence types (STs) were observed, with ST11, ST437 and ST340 [clonal complex 11 (CC11)] being the most prevalent (75 of isolates) observed in 10 states. bla(KPC-2) was associated with transposon Tn4401 ob' and in 36 this gene was found in IncN plasmids of 40 kb. In Brazil, the spread of bla(KPC-2) is occurring due to dispersion of Tn4401 ob', carried by IncN plasmids of 40 kb, and mainly the dissemination of CC11, with ST437 and ST11 playing an important role. |
BibTeX:@article{Pereira2013, author = {Pereira, P. S. and de Araujo, C. F. M. and Seki, L. M. and Zahner, V. and Carvalho-Assef, A. P. D. and Asensi, M. D.}, title = {Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340)}, journal = {Journal of Antimicrobial Chemotherapy}, publisher = {Oxford Univ Press}, year = {2013}, volume = {68}, number = {2}, pages = {312--316}, doi = {10.1093/jac/dks396}} |
| Petito RB, Amadeu TP, Pascarelli BMO, Jardim MR, Vital RT, Antunes SL and Sarno EN (), "Transforming Growth Factor-beta 1 May Be a Key Mediator of the Fibrogenic Properties of Neural Cells in Leprosy", Journal of Neuropathology and Experimental Neurology. [DOI] |
| Abstract: Fibrosis is the main cause of irreversible nerve damage in leprosy. Phenotypic changes in Mycobacterium leprae (ML)-infected Schwann cells (SCs) have been suggested to mediate this process. We found that SC line cultures stimulated with ML upregulated transforming growth factor-beta 1 (TGF-beta 1), and that TGF-beta 1 or ML induced increased numbers of alpha-smooth muscle actin (alpha-SMA)-positive cells with characteristic stress fibers. Mycobacterium leprae and TGF-beta 1 also induced increased type I collagen and fibronectin mRNA and secretion and augmented mRNA levels of SOX9 and ZEB1, which are involved in the epithelial-mesenchymal transition. These effects could be inhibited by the TGF-beta 1 type I receptor (ALK5) inhibitor, SB-431542. In nerve biopsies from leprosy-infected patients with varying grades of fibrosis (n = 11), type I and III collagen and fibronectin were found in the endoneurium and perineurium, alpha-SMA-positive cells filled the fibrotic perineurium but not the endoneurium, and CD34-positive fibroblasts predominated in the endoneurium. Results of transcriptional studies of 3 leprosy nerves and 5 controls were consistent with these data, but alpha-SMA and other mRNA levels were not different from those in the control samples. Our findings suggest that TGF-beta 1 may orchestrate events, including reprogramming of the SC phenotype, leading to transdifferentiation, connective tissue cell expansion, and fibrogenesis in the evolution of leprosy nerve lesions during some evolutionary stages. |
BibTeX:@article{Petito, author = {Petito, R. B. and Amadeu, T. P. and Pascarelli, B. M. O. and Jardim, M. R. and Vital, R. T. and Antunes, S. L. and Sarno, E. N.}, title = {Transforming Growth Factor-beta 1 May Be a Key Mediator of the Fibrogenic Properties of Neural Cells in Leprosy}, journal = {Journal of Neuropathology and Experimental Neurology}, doi = {10.1097/NEN.0b013e31828bfc60}} |
| Pinheiro RO, de Oliveira EB, dos Santos G, da Silva GMS, Silva BJD, Teles RMB, Milagres A, Sarno EN, Dalcolmo MP and Sampaio EP (2013), "Different immunosuppressive mechanisms in multi-drug-resistant tuberculosis and non-tuberculous mycobacteria patients", Clinical and Experimental Immunology., February, 2013. Vol. 171(2), pp. 210-219. Wiley-blackwell. [DOI] |
| Abstract: Previous studies have demonstrated that cells from both multi-drug-resistant tuberculosis (MDR-TB) and non-tuberculous mycobacteria (NTM) patients respond poorly to mycobacterial antigens in vitro. In the prepercentage of CD4+CD25+- forkhead box protein 3 (FoxP3)+ and CD4+CD25+CD127- regulatory T (Treg) cells when compared to NR-TB. Increased numbers of Treg cells and interleukin (IL)-10 serum levels were detected in MDR-TB, whereas elevated serum transforming growth factor (TGF)-beta was found in the NTM group. Cells of MDR-TB patients stimulated with early secretory antigenic target (ESAT)-6, but not purified protein derivative (PPD), showed a lower frequency of CD4+/interferon (IFN)-?+ T cells and enhanced CD4+CD25+FoxP3+, CD4+CD25+CD127- and CD4+CD25+IL-10+ T cell population. In addition, increased IL-10 secretion was observed in cultured MDR-TB cells following ESAT-6 stimulation, but not in NR-TB or NTM patients. In vitro blockade of IL-10 or IL-10Ra decreased the CD4+CD25+FoxP3+ frequencies induced by ESAT-6 in MDR-TB, suggesting a role of IL-10 on impaired IFN-? responses seen in MDR-TB. Depletion of CD4+CD25+ T lymphocytes restored the capacity of MDR-TB T cells to respond to ESAT-6 in vitro, which suggests a potential role for Treg/T regulatory 1 cells in the pathogenesis of MDR-TB. Together, our results indicate that although the similarities in chronicity, NTM- and MDR-TB-impaired antigenic responses involve different mechanisms. |
BibTeX:@article{Pinheiro2013, author = {Pinheiro, R. O. and de Oliveira, E. B. and dos Santos, G. and da Silva, G. M. S. and Silva, B. J. D. and Teles, R. M. B. and Milagres, A. and Sarno, E. N. and Dalcolmo, M. P. and Sampaio, E. P.}, title = {Different immunosuppressive mechanisms in multi-drug-resistant tuberculosis and non-tuberculous mycobacteria patients}, journal = {Clinical and Experimental Immunology}, publisher = {Wiley-blackwell}, year = {2013}, volume = {171}, number = {2}, pages = {210--219}, doi = {10.1111/cei.12007}} |
| Sales AM, Campos DP, Hacker MA, Nery JAD, Duppre NC, Rangel E, Sarno EN and Penna MLF (2013), "Progression of leprosy disability after discharge: is multidrug therapy enough?", Tropical Medicine & International Health., September, 2013. Vol. 18(9), pp. 1145-1153. Wiley-blackwell. [DOI] |
| Abstract: OBJECTIVE To evaluate the risk factors related to worsening of physical disabilities after treatment discharge among patients with leprosy administered 12 consecutive monthly doses of multidrug therapy (MDT/WHO). METHODS Cohort study was carried out at the Leprosy Laboratory in Rio de Janeiro, Brazil. We evaluated patients with multibacillary leprosy treated (MDT/WHO) between 1997 and 2007. The Cox proportional hazards model was used to estimate the relationship between the onset of physical disabilities after release from treatment and epidemiological and clinical characteristics. RESULTS The total observation time period for the 368 patients was 1570 person-years (PY), averaging 4.3years per patient. The overall incidence rate of worsening of disability was 6.5/100PY. Among those who began treatment with no disability, the incidence rate of physical disability was 4.5/100PY. Among those who started treatment with Grade 1 or 2 disabilities, the incidence rate of deterioration was 10.5/100PY. The survival analysis evidenced that when disability grade was 1, the risk was 1.61 (95% CI: 1.02-2.56), when disability was 2, the risk was 2.37 (95% CI 1.35-4.16), and when the number of skin lesions was 15 or more, an HR=1.97 (95% CI: 1.07-3.63). Patients with neuritis showed a 65% increased risk of worsening of disability (HR=1.65 [95% CI: 1.08-2.52]). CONCLUSION Impairment at diagnosis was the main risk factor for neurological worsening after treatment/MDT. Early diagnosis and prompt treatment of reactional episodes remain the main means of preventing physical disabilities. |
BibTeX:@article{Sales2013, author = {Sales, A. M. and Campos, D. P. and Hacker, M. A. and Nery, J. A. D. and Duppre, N. C. and Rangel, E. and Sarno, E. N. and Penna, M. L. F.}, title = {Progression of leprosy disability after discharge: is multidrug therapy enough?}, journal = {Tropical Medicine & International Health}, publisher = {Wiley-blackwell}, year = {2013}, volume = {18}, number = {9}, pages = {1145--1153}, doi = {10.1111/tmi.12156}} |
| Silva CAM, Danelishvili L, McNamara M, Berredo-Pinho M, Bildfell R, Biet F, Rodrigues LS, Oliveira AV, Bermudez LE and Pessolani MCV (2013), "Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis", Infection and Immunity., July, 2013. Vol. 81(7), pp. 2645-2659. Amer Soc Microbiology. [DOI] |
| Abstract: This study examined the in vitro interaction between Mycobacterium leprae, the causative agent of leprosy, and human alveolar and nasal epithelial cells, demonstrating that M. leprae can enter both cell types and that both are capable of sustaining bacterial survival. Moreover, delivery of M. leprae to the nasal septum of mice resulted in macrophage and epithelial cell infection in the lung tissue, sustaining the idea that the airways constitute an important M. leprae entry route into the human body. Since critical aspects in understanding the mechanisms of infection are the identification and characterization of the adhesins involved in pathogen-host cell interaction, the nude mouse-derived M. leprae cell surface-exposed proteome was studied to uncover potentially relevant adhesin candidates. A total of 279 cell surface-exposed proteins were identified based on selective biotinylation, streptavidin-affinity purification, and shotgun mass spectrometry; 11 of those proteins have been previously described as potential adhesins. In vitro assays with the recombinant forms of the histone-like protein (Hlp) and the heparin-binding hemagglutinin (HBHA), considered to be major mycobacterial adhesins, confirmed their capacity to promote bacterial attachment to epithelial cells. Taking our data together, they suggest that the airway epithelium may act as a reservoir and/or portal of entry for M. leprae in humans. Moreover, our report sheds light on the potentially critical adhesins involved in M. leprae-epithelial cell interaction that may be useful in designing more effective tools for leprosy control. |
BibTeX:@article{Silva2013a, author = {Silva, C. A. M. and Danelishvili, L. and McNamara, M. and Berredo-Pinho, M. and Bildfell, R. and Biet, F. and Rodrigues, L. S. and Oliveira, A. V. and Bermudez, L. E. and Pessolani, M. C. V.}, title = {Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis}, journal = {Infection and Immunity}, publisher = {Amer Soc Microbiology}, year = {2013}, volume = {81}, number = {7}, pages = {2645--2659}, doi = {10.1128/IAI.00147-13}} |
| Silva D, Ponte CGG, Hacker MA and Antas PRZ (), "A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens", Acta Tropica. [DOI] |
| Abstract: In vitro stimulation of whole blood or isolated peripheral blood cells with specific antigens is used for several purposes. We sought to identify a reliable, reproducible, fast and feasible in vitro method to assess human cellular immune responses to Mycobacterium tuberculosis. In contrast to peripheral blood mononuclear cell (PBMC) culture, a whole blood assay (WBA) provides a more physiological environment, which may provide a broader assessment of serum biomarker, biosignature profiles. Twenty-three asymptomatic individuals with M. tuberculosis infection were recruited. Total cells from the WBA (diluted 1:3 in completed RPMI) and PBMC (2 x 10(5) cells/ml) plus M. tuberculosis Ag85A, Ag85B, ESAT-6 and Mycobacterium bovis 65 kDa were characterized by flow cytometry, then added in 96-well plates and on day 5 plasma and supernatants were harvested for detection of 17 cytokines by a Luminex array system. There was agreement between PBMC and WBA for IL-2, IL-5, IL-6, IL-7, IL-13, IFN-gamma, TNF-alpha, MCP-1 and MIP-1 beta. There was evidence toward higher IL-10 (p <= 0.049) and G-CSF (p <= 0.012) plasma production, and higher IL-1 beta (p <= 0.048), IL-4 (p <= 0.044), IL-12p70 (p <= 0.006), IL-17 (p <= 0.002) and GM-CSF (p <= 0.049) production for PBMC vs. WBA. Both methods provided virtually no reaction to the internal, negative control. Due to technical issues linked to data out of range, IL-8 data were not considered. These results suggest that, depending on the method employed, PBMC and/or WBA techniques provide fine conditions for the model proposed and thus whole blood cultures are well-suited low-cost proxy-measures during search for serum biomarkers. (c) 2013 Elsevier B.V. All rights reserved. |
BibTeX:@article{Silva, author = {Silva, D. and Ponte, C. G. G. and Hacker, M. A. and Antas, P. R. Z.}, title = {A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens}, journal = {Acta Tropica}, doi = {10.1016/j.actatropica.2013.04.002}} |
| Silva MR, Rocha AD, da Costa RR, de Alencar AP, de Oliveira VM, Fonseca AA, Sales ML, Issa MD, Filho PMS, Pereira OTV, dos Santos EC, Mendes RS, Ferreira AMD, Mota PMPC, Suffys PN and Guimaraes MDC (2013), "Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil", Memorias Do Instituto Oswaldo Cruz., May, 2013. Vol. 108(3), pp. 321-327. Fundaco Oswaldo Cruz. [DOI] |
| Abstract: In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Lowenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials. |
BibTeX:@article{Silva2013, author = {Silva, M. R. and Rocha, A. D. and da Costa, R. R. and de Alencar, A. P. and de Oliveira, V. M. and Fonseca, A. A. and Sales, M. L. and Issa, M. D. and Filho, P. M. S. and Pereira, O. T. V. and dos Santos, E. C. and Mendes, R. S. and Ferreira, A. M. D. and Mota, P. M. P. C. and Suffys, P. N. and Guimaraes, M. D. C.}, title = {Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil}, journal = {Memorias Do Instituto Oswaldo Cruz}, publisher = {Fundaco Oswaldo Cruz}, year = {2013}, volume = {108}, number = {3}, pages = {321--327}, doi = {10.1590/S0074-02762013000300010}} |
| Vinhas SA, Palaci M, Marques HS, de Aguiar PPL, Ribeiro FK, Peres RL, Dietze R, Gomes HM, Suffys PN, Golub JE, Riley LW and Maciel ELN (2013), "Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil", Tuberculosis., March, 2013. Vol. 93(2), pp. 207-212. Churchill Livingstone. [DOI] |
| Abstract: Mycobacterium tuberculosis (Mtb) strains designated as RDRio are responsible for a large cluster of new cases of tuberculosis (TB) in Rio de Janeiro. They were previously shown to be associated with severe manifestations of TB. Here, we used three genotyping methods (IS6110 RFLP, spoligotyping, and multiplex PCR) to characterize RDRio and non-RDRio strains from the metropolitan area of Vitoria, State of Espirito Santo in southeast Brazil to determine strain diversity and transmission patterns. Strains with identical IS6110 RFLP patterns were considered to belong to a cluster indicative of recent transmission. Between 2000 and 2010, we identified 5470 new TB patients and genotyped 981 Mtb strains. Of these, 376 (38%) were RDRio. By RFLP, 180 (48%) of 376 RDRio strains and 235 (40%) of 593 non-RDRio strains belonged to RFLP cluster pattern groups (p = 0.023). Simpson's diversity index based on RFLP patterns was 0.96 for RDRio and 0.98 for non-RDRio strains. Thus, although RDRio strains appear to be comprised of a fewer number of RFLP genotypes, they represent a heterogeneous group. While TB cases caused by RDRio appear more likely to be due to recent transmission than cases caused by non-RDRio strains, the difference is small. These observations suggest that factors other than inherent biological characteristic of RDRio lineages are more important in determining recent transmission, and that public health measures to interrupt new transmissions need to be emphasized for TB control in Vitoria. (C) 2012 Elsevier Ltd. All rights reserved. |
BibTeX:@article{Vinhas2013, author = {Vinhas, S. A. and Palaci, M. and Marques, H. S. and de Aguiar, P. P. L. and Ribeiro, F. K. and Peres, R. L. and Dietze, R. and Gomes, H. M. and Suffys, P. N. and Golub, J. E. and Riley, L. W. and Maciel, E. L. N.}, title = {Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil}, journal = {Tuberculosis}, publisher = {Churchill Livingstone}, year = {2013}, volume = {93}, number = {2}, pages = {207--212}, doi = {10.1016/j.tube.2012.09.001}} |
| Vital RT, Illarramendi X, Antunes SLG, Nascimento M, Nery JAD, Nascimento O, Sarno EN and Jardim MR (2013), "Isolated median neuropathy as the first symptom of leprosy", Muscle & Nerve., August, 2013. Vol. 48(2), pp. 179-184. Wiley-blackwell. [DOI] |
| Abstract: Introduction: Focal peripheral neuropathy of the median nerve is mainly caused by a traumatic event or pressure, but it may also be produced by systemic illnesses. Among the latter, leprosy is a rare cause. Methods: Six cases of isolated median neuropathy as the first sign of leprosy were selected from patients with an exclusively neurological complaint as the initial symptom. The patients, evaluated at the National Leprosy Reference Center in Rio de Janeiro, Brazil, followed routine and specialized procedures. Results: Three of the patients had pure neural leprosy, and 3 had skin lesions. Clinical median nerve function impairment was confirmed by neurophysiological testing and histopathology. Both mononeuritis and mononeuritis multiplex were observed. Conclusions: This case series demonstrates an additional form of presentation of leprosy, which, if not diagnosed and treated in time, may lead to permanent disability. |
BibTeX:@article{Vital2013, author = {Vital, R. T. and Illarramendi, X. and Antunes, S. L. G. and Nascimento, M. and Nery, J. A. D. and Nascimento, O. and Sarno, E. N. and Jardim, M. R.}, title = {Isolated median neuropathy as the first symptom of leprosy}, journal = {Muscle & Nerve}, publisher = {Wiley-blackwell}, year = {2013}, volume = {48}, number = {2}, pages = {179--184}, doi = {10.1002/mus.23731}} |
| Zumarraga MJ, Arriaga C, Barandiaran S, Cobos-Marin L, de Waard J, Estrada-Garcia I, Figueiredo T, Figueroa A, Gimenez F, Gomes HM, Gonzalez-y-Merchand JA, Macias A, Milian-Suazo F, Rodriguez CAR, Santillan MA, Suffys PN, Trangoni MD, Zarraga AM and Cataldi A (2013), "Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American Countries", Research In Veterinary Science., February, 2013. Vol. 94(1), pp. 9-21. Elsevier Sci Ltd. [DOI] |
| Abstract: Spoligotyping is the most frequently used method for genotyping isolates of Mycobacterium bovis worldwide. In the current work, we compared spoligotypes from 1684 M. bovis isolates from Argentina (816), Brazil (412), Chile (66), Mexico (274) and Venezuela (116), obtained from cattle, humans, pigs, wild boars, farmed deer, goats, buffaloes, cats, and wild animals. A total of 269 different spoligotypes were found: 142 (8.4%) isolates presented orphan spoligotypes, whereas 1542 (91.6%) formed 113 different clusters. In cattle, SB0140 was the most representative spoligotype with 355 (24.6%) isolates, followed by SB0121 with 149 (10.3%) isolates. Clustering of spoligotypes ranged from 95.2% in Argentina to 85.3% in Mexico. Orphan spoligotypes were also variable, ranging from 23.7% in Mexico to 4.1% in Brazil. A large proportion of spoligotypes were common to the neighboring countries Argentina, Brazil and Chile. In conclusion, despite the diversity of spoligotypes found in the five countries studied, there are major patterns that predominate in these neighboring countries. These clusters may reflect a long-lasting active transmission of bovine tuberculosis or common historical origins of infection. (C) 2012 Elsevier Ltd. All rights reserved. |
BibTeX:@article{Zumarraga2013, author = {Zumarraga, M. J. and Arriaga, C. and Barandiaran, S. and Cobos-Marin, L. and de Waard, J. and Estrada-Garcia, I. and Figueiredo, T. and Figueroa, A. and Gimenez, F. and Gomes, H. M. and Gonzalez-y-Merchand, J. A. and Macias, A. and Milian-Suazo, F. and Rodriguez, C. A. R. and Santillan, M. A. and Suffys, P. N. and Trangoni, M. D. and Zarraga, A. M. and Cataldi, A.}, title = {Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American Countries}, journal = {Research In Veterinary Science}, publisher = {Elsevier Sci Ltd}, year = {2013}, volume = {94}, number = {1}, pages = {9--21}, doi = {10.1016/j.rvsc.2012.07.012}} |
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