Área de PDI em Pesquisa, Desenvolvimento e Inovação em Malária, Toxoplasmose e Outras Protozooses 2013
Veja, abaixo, a relação de artigos científicos publicados pelo IOC, na referida Área Temática, organizados em ordem alfabética crescente:
| Bahia AC, Oliveira JHM, Kubota MS, Araujo HRC, Lima JBP, Rios-Velasquez CM, Lacerda MVG, Oliveira PL, Traub-Cseko YM and Pimenta PFP (2013), "The Role of Reactive Oxygen Species in Anopheles aquasalis Response to Plasmodium vivax Infection", Plos One., February, 2013. Vol. 8(2), pp. e57014. Public Library Science. [DOI] |
| Abstract: Malaria affects millions of people worldwide and hundreds of thousands of people each year in Brazil. The mosquito Anopheles aquasalis is an important vector of Plasmodium vivax, the main human malaria parasite in the Americas. Reactive oxygen species (ROS) have been shown to have a role in insect innate immune responses as a potent pathogen-killing agent. We investigated the mechanisms of free radicals modulation after A. aquasalis infection with P. vivax. ROS metabolism was evaluated in the vector by studying expression and activity of three key detoxification enzymes, one catalase and two superoxide dismutases (SOD3A and SOD3B). Also, the involvement of free radicals in the mosquito immunity was measured by silencing the catalase gene followed by infection of A. aquasalis with P. vivax. Catalase, SOD3A and SOD3B expression in whole A. aquasalis were at the same levels of controls at 24 h and upregulated 36 h after ingestion of blood containing P. vivax. However, in the insect isolated midgut, the mRNA for these enzymes was not regulated by P. vivax infection, while catalase activity was reduced 24 h after the infectious meal. RNAi-mediated silencing of catalase reduced enzyme activity in the midgut, resulted in increased P. vivax infection and prevalence, and decreased bacterial load in the mosquito midgut. Our findings suggest that the interactions between A. aquasalis and P. vivax do not follow the model of ROS-induced parasite killing. It appears that P. vivax manipulates the mosquito detoxification system in order to allow its own development. This can be an indirect effect of fewer competitive bacteria present in the mosquito midgut caused by the increase of ROS after catalase silencing. These findings provide novel information on unique aspects of the main malaria parasite in the Americas interaction with one of its natural vectors. |
BibTeX: @article{Bahia2013, author = {Bahia, A. C. and Oliveira, J. H. M. and Kubota, M. S. and Araujo, H. R. C. and Lima, J. B. P. and Rios-Velasquez, C. M. and Lacerda, M. V. G. and Oliveira, P. L. and Traub-Cseko, Y. M. and Pimenta, P. F. P.}, title = {The Role of Reactive Oxygen Species in Anopheles aquasalis Response to Plasmodium vivax Infection}, journal = {Plos One}, publisher = {Public Library Science}, year = {2013}, volume = {8}, number = {2}, pages = {e57014}, doi = {10.1371/journal.pone.0057014} } |
| Da Silva KS, Pinto ID, Leite GR, das Virgens TM, dos Santos CB and Falqueto A (2013), "Ecology of Anopheline Mosquitoes (Diptera: Culicidae) in the Central Atlantic Forest Biodiversity Corridor, Southeastern Brazil", Journal of Medical Entomology., January, 2013. Vol. 50(1), pp. 24-30. Entomological Soc Amer. [DOI] |
| Abstract: Knowledge of the fauna composition of anopheline mosquitoes, their ecological aspects and behavior, and influence of climatic variables on their population dynamics can help in understanding the transmission of Plasmodium parasites and thus develop more efficient strategies for the control of malaria. In the Central Atlantic Forest Biodiversity Corridor, southeastern Brazil, foci of introduced malaria have been reported among people returning from the Amazon region, north Brazil. Our objective was to evaluate and compare the anopheline fauna from a preserved environment and an adjacent peridomiciliary modified environment at the Central Atlantic Forest Biodiversity Corridor. We collected anopheline mosquitoes on a monthly basis from June 2004 to May 2006 from both these environments to understand the ecological aspects and their association with the occurrence of malaria. We captured 5,491 anopheline mosquitoes belonging to two subgenera and 11 species and studied the correlations between anopheline mosquito species and climatic variables. We considered Anopheles darlingi (Root) as the principal malaria vector and Anopheles albitarsis s. l. (Arribalzaga) as the secondary vector. |
BibTeX: @article{Silva2013, author = {Da Silva, K. S. and Pinto, I. D. and Leite, G. R. and das Virgens, T. M. and dos Santos, C. B. and Falqueto, A.}, title = {Ecology of Anopheline Mosquitoes (Diptera: Culicidae) in the Central Atlantic Forest Biodiversity Corridor, Southeastern Brazil}, journal = {Journal of Medical Entomology}, publisher = {Entomological Soc Amer}, year = {2013}, volume = {50}, number = {1}, pages = {24--30}, doi = {10.1603/ME11219} } |
| De Moura FL, Amendoeira MRR, Bastos OMP, de Mattos DPBG, Fonseca ABM, Nicolau JL, das Neves LB and Millar PR (2013), "Prevalence and risk factors for Toxoplasma gondii infection among pregnant and postpartum women attended at public healthcare facilities in the City of Niteroi, State of Rio de Janeiro, Brazil", Revista Da Sociedade Brasileira De Medicina Tropical., March, 2013. Vol. 46(2), pp. 200-207. Soc Brasileira Medicina Tropical. [DOI] |
| Abstract: Introduction: To determine the prevalence of immunoglobulin G (IgG) and immunoglobulin M (IgM) anti-Toxoplasma gondii antibodies among pregnant and postpartum women attended within the public healthcare system in Niteroi, State of Rio de Janeiro, and to detect possible exposure factors associated with T gondii infection in this population. Methods: IgM and IgG anti- T gondii antibodies were investigated in 276 pregnant and 124 postpartum women by using the indirect immunofluorescence (IFAT) and immunoenzymatic assay (ELISA) techniques. The participants were selected by convenience sampling. All these 400 patients filled out a free and informed consent statement, answered an epidemiological questionnaire and were informed about the disease. Results: Among the 400 samples analyzed, 234 (58.5%) were reactive to IgG anti-IT gondii antibodies, according to the IFAT and/or ELISA assay. One pregnant woman was found to be reactive to IgM anti- T gondii antibodies, with an intermediate IgG avidity test. Risk factor analysis showed that seropositivity was significantly associated (p<0.05) with age, contact with cats and presence of rodents at home. Through a logistic regression model, these associations were confirmed for age and contact with cats, while education at least of the high school level was found to be a protective factor. Conclusions: The prevalence rate of IgG anti-IT gondii antibodies in the City of Niteroi was high and the risk factors for infection detected after multivariate analysis were: age over 30 years, contact with cats and education levels lower than university graduate level. |
BibTeX: @article{Moura2013, author = {De Moura, F. L. and Amendoeira, M. R. R. and Bastos, O. M. P. and de Mattos, D. P. B. G. and Fonseca, A. B. M. and Nicolau, J. L. and das Neves, L. B. and Millar, P. R.}, title = {Prevalence and risk factors for Toxoplasma gondii infection among pregnant and postpartum women attended at public healthcare facilities in the City of Niteroi, State of Rio de Janeiro, Brazil}, journal = {Revista Da Sociedade Brasileira De Medicina Tropical}, publisher = {Soc Brasileira Medicina Tropical}, year = {2013}, volume = {46}, number = {2}, pages = {200--207}, doi = {10.1590/0037-8682-1613-2013} } |
| Fajardo HV, D'avila S, Bastos RR, Cyrino CD, Detoni MD, Garcia JL, das Neves LB, Nicolau JL and Amendoeira MRR (2013), "Seroprevalence and risk factors of toxoplasmosis in cattle from extensive and semi-intensive rearing systems at Zona da Mata, Minas Gerais state, Southern Brazil", Parasites & Vectors., June, 2013. Vol. 6, pp. 191. Biomed Central Ltd. [DOI] |
| Abstract: Background: Concerning the infection of humans by T. gondii, limited efforts have been directed to the elucidation of the role of horizontal transmission between hosts. One of the main routes of transmission from animals to humans occurs through the ingestion of raw or insufficiently cooked meat. However, even though the detection of T. gondii in meat constitutes an important short-term measure, control strategies can only be accomplished by a deeper understanding of the epidemiology of toxoplasmosis. The present study aimed to investigate the seroprevalence of toxoplasmosis in cattle from Zona da Mata, Minas Gerais, Brazil, and to identify associated risk factors, through an epidemiological investigation. Methods: The animals studied (Bos indicus, breed Nelore or Gir) were reared in the Zona da Mata micro-region and killed at a commercial slaughterhouse at Juiz de Fora, Minas Gerais state. The animals came from 53 cattle farms with extensive (predominantly pasture feeding management) or semi-intensive (food management based on grazing, salt mineral and feed supplementation) rearing systems. Blood samples were collected from 1200 animals, and assigned to Indirect Fluorescent Antibody Test. Results: When analyzing IgG anti-T. gondii we found an overall seroprevalence of 2.68%. In Brazil prevalences vary from 1.03% to 60%. Although in the present study, the seroprevalence per animal is considered low compared to those observed in other studies, we found out that of the 53 farms analyzed, 17 (34.69%) had one or more positive cattle. It is a considerable percentage, suggesting that the infection is well distributed through the Zona da Mata region. The results of the epidemiological investigation showed that the main risk factors of Toxoplasma gondii infection are related to animal management and to the definive host. There was a relationship between the number of seropositive cattle and the presence and number of resident cats, presence and number of stray cats, presence of cats walking freely, rat control by using cats and feed storage. Conclusion: These results may contribute to the development of preventive strategies in Brazil and other developing countries were extensive and semi-intensive cattle rearing systems are very widespread and the efforts to control this important zoonotic disease have attained little success. |
BibTeX: @article{Fajardo2013, author = {Fajardo, H. V. and D'avila, S. and Bastos, R. R. and Cyrino, C. D. and Detoni, M. D. and Garcia, J. L. and das Neves, L. B. and Nicolau, J. L. and Amendoeira, M. R. R.}, title = {Seroprevalence and risk factors of toxoplasmosis in cattle from extensive and semi-intensive rearing systems at Zona da Mata, Minas Gerais state, Southern Brazil}, journal = {Parasites & Vectors}, publisher = {Biomed Central Ltd}, year = {2013}, volume = {6}, pages = {191}, doi = {10.1186/1756-3305-6-191} } |
| Gomes LR, Totino PRR, Sanchez MCA, Daniel EPdSK, Macedo CSd, Fortes F, Coura JR, Santi SMD, Werneck GL, Suárez-Mutis MC, Ferreira-da-Cruz MdF and Daniel-Ribeiro CT (2013), "Asymptomatic infection in individuals from the municipality of Barcelos (Brazilian Amazon) is not associated with the anti-Plasmodium falciparum glycosylphosphatidylinositol antibody response.", Mem Inst Oswaldo Cruz., Sep, 2013. Vol. 108(6), pp. 796-800. [DOI] |
| Abstract: Anti-glycosylphosphatidylinositol (GPI) antibodies (Abs) may reflect and mediate, at least partially, anti-disease immunity in malaria by neutralising the toxic effect of parasitic GPI. Thus, we assessed the anti-GPI Ab response in asymptomatic individuals living in an area of the Brazilian Amazon that has a high level of malaria transmission. For comparative purposes, we also investigated the Ab response to a crude extract prepared from Plasmodium falciparum, the merozoite surface protein (MSP)3 antigen of P. falciparum and the MSP 1 antigen of Plasmodium vivax (PvMSP1-19) in these individuals and in Angolan patients with acute malaria. Our data suggest that the Ab response against P. falciparum GPI is not associated with P. falciparum asymptomatic infection in individuals who have been chronically exposed to malaria in the Brazilian Amazon. However, this Ab response could be related to ongoing parasitaemia (as was previously shown) in the Angolan patients. In addition, our data show that PvMSP1-19may be a good marker antigen to reflect previous exposure to Plasmodium in areas that have a high transmission rate of P. vivax. |
BibTeX: @article{Gomes2013, author = {Gomes, Larissa Rodrigues and Totino, Paulo Renato Rivas and Sanchez, Maria Carmen Arroyo and Daniel, Elsa Paula da Silva Kaingona and Macedo, Cristiana Santos de and Fortes, Filomeno and Coura, José Rodrigues and Santi, Silvia Maria Di and Werneck, Guilherme Loureiro and Suárez-Mutis, Martha Cecilia and Ferreira-da-Cruz, Maria de Fátima and Daniel-Ribeiro, Cláudio Tadeu}, title = {Asymptomatic infection in individuals from the municipality of Barcelos (Brazilian Amazon) is not associated with the anti-Plasmodium falciparum glycosylphosphatidylinositol antibody response.}, journal = {Mem Inst Oswaldo Cruz}, year = {2013}, volume = {108}, number = {6}, pages = {796--800}, url = {http://dx.doi.org/10.1590/0074-0276108062013018}, doi = {10.1590/0074-0276108062013018} } |
| Jaeger LH, Taglioretti V, Fugassa MH, Dias O, Neto J and Iniguez AM (2013), "Paleoparasitological results from XVIII century human remains from Rio de Janeiro, Brazil", Acta Tropica., March, 2013. Vol. 125(3), pp. 282-286. Elsevier Science Bv. [DOI] |
| Abstract: Paleoparasitological studies of the Brazilian colonial period are scarce. A paleoparasitological analysis was performed on human remains from the archeological site Praca XV Cemetery in Rio de Janeiro, dating from the early 18th to 19th Centuries. The samples were obtained from the Institute of the Brazilian Archaeology collection, and showed evidence of washing and brushing. Sediments were extracted from sacral foramina by scraping. Sediments from skulls were used as negative paleoparasitological controls. Spontaneous sedimentation method was performed prior to microscopic analysis. The results revealed that 8 of 10 individuals were infected with intestinal helminths and/or protozoa. Eggs of the nematodes Trichuris sp. and Ascaris sp. as well as a single taeniid egg were found. Protozoa cysts suggestive of Entamoeba sp. were also observed. Trichuris sp. was the most frequent and abundant parasite, found in 70% of individuals (26 eggs). The study showed the importance of analysis of sediment from human remains preserved in museum or scientific collections, even those subjected to a curating procedure. The levels of infection revealed here should be considered underestimations. This is the first paleoparasitological study from Rio de Janeiro city for the Brazilian colonial period and the first report of human Taenia sp. in the New World. (c) 2012 Elsevier B.V. All rights reserved. |
BibTeX: @article{Jaeger2013, author = {Jaeger, L. H. and Taglioretti, V. and Fugassa, M. H. and Dias, O. and Neto, J. and Iniguez, A. M.}, title = {Paleoparasitological results from XVIII century human remains from Rio de Janeiro, Brazil}, journal = {Acta Tropica}, publisher = {Elsevier Science Bv}, year = {2013}, volume = {125}, number = {3}, pages = {282--286}, doi = {10.1016/j.actatropica.2012.11.007} } |
| Luz TCB, Suarez-Mutis MC, Miranda ES, Moritz AFE, Freitas LF, Brasil JD and Osorio-de-Castro CGS (2013), "Uncomplicated malaria among pregnant women in the Brazilian Amazon: Local barriers to prompt and effective case management", Acta Tropica., February, 2013. Vol. 125(2), pp. 137-142. Elsevier Science Bv. [DOI] |
| Abstract: Malaria in pregnancy is associated with increased risks of maternal anemia, spontaneous abortion, low birth weight, premature delivery and other adverse effects on health. In Brazil, disease transmission is highly concentrated in the multi-state region that constitutes the Brazilian Amazon (more than 99% of all cases). This study, conducted between the first bimesters of 2007 and 2008, aims to identify the local barriers to prompt and effective case management of malaria in pregnancy and was carried out in health facilities located in three endemic municipalities of the Brazilian Amazon (Manaus, Presidente Figueiredo and Porto Velho). The study design combined both qualitative and quantitative descriptive methods. The qualitative design involved semi-structured interviews with health personnel who routinely deal with malaria care. The quantitative design involved a review of medical records of pregnant women in the visited health facilities. Additionally, data were abstracted from SIVEP-Malaria Epidemiological Surveillance Information System (Brasil, 2007) and Primary Care Information System (SIAB) databases. Flaws were detected in diagnosis (only 6.8% of women tested for malaria) and treatment (for Plasmodium falciparum infections, only 44.8% of patients received recommended first-line therapy; 10.2% of prescription presented treatments were not found in national guideline and 7.3% of the prescriptions for Plasmodium vivax and 17.9% of the prescriptions for P. falciparum were not sanctioned by the official guidelines). Training (only 37.3% had had some training), knowledge and counseling were also sub-optimal. These results indicated the need to improve the health-worker performance through training. Close supervision and feedback on the health-worker performance are also needed. These findings also highlighted the need to put into practice a series of government recommendations that encourage close collaboration between the National Malaria Control Program and Primary Health Care actions in order to achieve safer pregnancies. (c) 2012 Elsevier B.V. All rights reserved. |
BibTeX: @article{Luz2013, author = {Luz, T. C. B. and Suarez-Mutis, M. C. and Miranda, E. S. and Moritz, A. F. E. and Freitas, L. F. and Brasil, J. D. and Osorio-de-Castro, C. G. S.}, title = {Uncomplicated malaria among pregnant women in the Brazilian Amazon: Local barriers to prompt and effective case management}, journal = {Acta Tropica}, publisher = {Elsevier Science Bv}, year = {2013}, volume = {125}, number = {2}, pages = {137--142}, doi = {10.1016/j.actatropica.2012.11.004} } |
| Menezes BF, Vigoder FM, Peixoto AA, Varaldi J and Bitner-Mathe BC (2013), "The influence of male wing shape on mating success in Drosophila melanogaster", Animal Behaviour., June, 2013. Vol. 85(6), pp. 1217-1223. Academic Press Ltd- Elsevier Science Ltd. [DOI] |
| Abstract: Drosophila melanogaster males court females through a series of steps. In one of these steps, the male vibrates his wings to produce a courtship song that is species specific. Variation in the shape of Drosophila wings has been described among populations and species, but it is still unknown whether this variation influences the courtship process. In our laboratory, replicate strains were obtained from a natural population of D. melanogaster by artificial selection for rounded or elongated wing shapes. We used those strains and a nonselected strain to test the influence of wing shape on male mating success. We observed significantly higher success by males from strains with elongated wings when competing with those with rounded or nonselected wings, regardless of the female type. In addition, smaller males were more successful. We also recorded the courtship songs produced by males of different wing shape strains. We detected some variation among the courtship song produced by males depending on the original strain. However, this difference did not fully explain the success of males with elongated wings. Our results therefore show that wing shape has to be considered as influencing male mating success in Drosophila. (C) 2013 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved. |
BibTeX: @article{Menezes2013, author = {Menezes, B. F. and Vigoder, F. M. and Peixoto, A. A. and Varaldi, J. and Bitner-Mathe, B. C.}, title = {The influence of male wing shape on mating success in Drosophila melanogaster}, journal = {Animal Behaviour}, publisher = {Academic Press Ltd- Elsevier Science Ltd}, year = {2013}, volume = {85}, number = {6}, pages = {1217--1223}, doi = {10.1016/j.anbehav.2013.03.008} } |
| Moreno M, Bickersmith S, Harlow W, Hildebrandt J, McKeon SN, Silva-do-Nascimento TF, Loaiza JR, Ruiz F, Lourenco-de-Oliveira R, Sallum MAM, Bergo ES, Fritz GN, Wilkerson RC, Linton YM, Juri MJD, Rangel Y, Povoa MM, Gutierrez-Builes LA, Correa MM and Conn JE (2013), "Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns", Parasites & Vectors., February, 2013. Vol. 6, pp. 47. Biomed Central Ltd. [DOI] |
| Abstract: Background: The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatus s.s., Anopheles halophylus and the putative species Anopheles triannulatus C were investigated. Methods: The mitochondrial COI gene, the nuclear white gene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results: Each data set analyzed separately yielded a different topology but none provided evidence for the separation of An. halophylus and An. triannulatus C, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the white gene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s. l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses, strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean + Amazon Delta (clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion: Combination of the three molecular markers provided the best resolution for differentiation within An. triannulatus s. s. and An. halophylus and C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s. l. The potential implications for malaria epidemiology remain to be investigated. |
BibTeX: @article{Moreno2013, author = {Moreno, M. and Bickersmith, S. and Harlow, W. and Hildebrandt, J. and McKeon, S. N. and Silva-do-Nascimento, T. F. and Loaiza, J. R. and Ruiz, F. and Lourenco-de-Oliveira, R. and Sallum, M. A. M. and Bergo, E. S. and Fritz, G. N. and Wilkerson, R. C. and Linton, Y. M. and Juri, M. J. D. and Rangel, Y. and Povoa, M. M. and Gutierrez-Builes, L. A. and Correa, M. M. and Conn, J. E.}, title = {Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns}, journal = {Parasites & Vectors}, publisher = {Biomed Central Ltd}, year = {2013}, volume = {6}, pages = {47}, doi = {10.1186/1756-3305-6-47} } |
| Pratt-Riccio LR, Perce-da-Silva DdS, Lima-Junior JdC, Theisen M, Santos F, Daniel-Ribeiro CT, de Oliveira-Ferreira J and Banic DM (2013), "Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil.", Mem Inst Oswaldo Cruz., Jun, 2013. Vol. 108(4), pp. 523-528. [DOI] |
| Abstract: The genetic diversity displayed by Plasmodium falciparum, the most deadly Plasmodium species, is a significant obstacle for effective malaria vaccine development. In this study, we identified genetic polymorphisms in P. falciparum glutamate-rich protein (GLURP), which is currently being tested in clinical trials as a malaria vaccine candidate, from isolates found circulating in the Brazilian Amazon at variable transmission levels. The study was performed using samples collected in 1993 and 2008 from rural villages situated near Porto Velho, in the state of Rondônia. DNA was extracted from 126 P. falciparum-positive thick blood smears using the phenol-chloroform method and subjected to a nested polymerase chain reaction protocol with specific primers against two immunodominant regions of GLURP, R0 and R2. Only one R0 fragment and four variants of the R2 fragment were detected. No differences were observed between the two time points with regard to the frequencies of the fragment variants. Mixed infections were uncommon. Our results demonstrate conservation of GLURP-R0 and limited polymorphic variation of GLURP-R2 in P. falciparum isolates from individuals living in Porto Velho. This is an important finding, as genetic polymorphisms in B and T-cell epitopes could have implications for the immunological properties of the antigen. |
BibTeX: @article{Pratt-Riccio2013, author = {Pratt-Riccio, Lilian Rose and Perce-da-Silva, Daiana de Souza and Lima-Junior, Josué da Costa and Theisen, Michael and Santos, Fátima and Daniel-Ribeiro, Cláudio Tadeu and de Oliveira-Ferreira, Joseli and Banic, Dalma Maria}, title = {Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil.}, journal = {Mem Inst Oswaldo Cruz}, year = {2013}, volume = {108}, number = {4}, pages = {523--528}, url = {http://dx.doi.org/10.1590/S0074-02762013000400022}, doi = {10.1590/S0074-02762013000400022} } |
| Totino PRR, Magalhaes AD, Silva LA, Banic DM, Daniel-Ribeiro CT and Ferreira-da-Cruz MD (2010), "Apoptosis of non-parasitized red blood cells in malaria: a putative mechanism involved in the pathogenesis of anaemia", Malaria Journal., December, 2010. Vol. 9, pp. 350. Biomed Central Ltd. [DOI] |
| Abstract: Background: Severe anaemia is a common complication of Plasmodium falciparum malaria in hyperendemic regions. Premature elimination of non-parasitized red blood cells (nRBC) has been considered as one mechanism involved in the genesis of severe malaria anaemia. It has been reported that apoptosis can occur in RBC and, consequently, this cell death process could contribute to anaemia. This study was performed to evaluate the susceptibility of nRBC to apoptosis in a malaria anaemia murine model. Methods: Balb/c mice were intraperitonially inoculated with 1 x 10(6) P. yoelii 17XL parasitized RBC (pRBC) and, then, parasitaemia and anaemia were monitored. Apoptosis in both pRBC and nRBC was assessed during early and late phases of infection by flow cytometry using Syto 16 and annexin V-PE double staining and forward scatter measurement. Results: As expected, experimental infection of Balb/c mice with Plasmodium yoelii 17XL parasites was characterized by progressive increase of parasitaemia and acute anaemia, leading to death. Flow cytometry analysis showed that a number of pRBC was in the apoptotic process. It was noteworthy that the increase of nRBC apoptosis levels occurred in the late phase of infection, when anaemia degree was notably accentuated, while no significant alteration was observed in the early phase. Conclusion: The increased levels of nRBC apoptosis herein firstly reported, in malaria infection could represent a putative mechanism worsening the severity of malarial anaemia. |
BibTeX: @article{Totino2010, author = {Totino, P. R. R. and Magalhaes, A. D. and Silva, L. A. and Banic, D. M. and Daniel-Ribeiro, C. T. and Ferreira-da-Cruz, M. D.}, title = {Apoptosis of non-parasitized red blood cells in malaria: a putative mechanism involved in the pathogenesis of anaemia}, journal = {Malaria Journal}, publisher = {Biomed Central Ltd}, year = {2010}, volume = {9}, pages = {350}, doi = {10.1186/1475-2875-9-350} } |
| Totino PRR, Pinna RA, De-Oliveira ACAX, Banic DM, Daniel-Ribeiro CT and Ferreira-da-Cruz MD (2013), "Apoptosis of non-parasitised red blood cells in Plasmodium yoelii malaria", Memorias Do Instituto Oswaldo Cruz., September, 2013. Vol. 108(6), pp. 686-690. Fundaco Oswaldo Cruz. [DOI] |
| Abstract: Recently, while studying erythrocytic apoptosis during Plasmodium yoelii infection, we observed an increase in the levels of non-parasitised red blood cell (nRBC) apoptosis, which could be related to malarial anaemia. Therefore, in the present study, we attempted to investigate whether nRBC apoptosis is associated with the peripheral RBC count, parasite load or immune response. To this end, BALB/c mice were infected with P. yoelii 17XL and nRBC apoptosis, number of peripheral RBCs, parasitaemia and plasmatic levels of cytokines, nitric oxide and anti-RBC antibodies were evaluated at the early and late stages of anaemia. The apoptosis of nRBCs increased at the late stage and was associated with parasitaemia, but not with the intensity of the immune response. The increased percentage of nRBC apoptosis that was observed when anaemia was accentuated was not related to a reduction in peripheral RBCs. We conclude that nRBC apoptosis in P. yoelii malaria appears to be induced in response to a high parasite load. Further studies on malaria models in which acute anaemia develops during low parasitaemia are needed to identify the potential pathogenic role of nRBC apoptosis. |
BibTeX: @article{Totino2013, author = {Totino, P. R. R. and Pinna, R. A. and De-Oliveira, A. C. A. X. and Banic, D. M. and Daniel-Ribeiro, C. T. and Ferreira-da-Cruz, M. D.}, title = {Apoptosis of non-parasitised red blood cells in Plasmodium yoelii malaria}, journal = {Memorias Do Instituto Oswaldo Cruz}, publisher = {Fundaco Oswaldo Cruz}, year = {2013}, volume = {108}, number = {6}, pages = {686--690}, doi = {10.1590/0074-0276108062013003} } |
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